摘要
目的:探讨在大鼠动脉粥样硬化(AS)形成过程中凝血酶活化的纤维蛋白溶解抑制剂(TAFI)活性水平变化及作用机制。方法:将40只健康的雄性Wistar大鼠随机分为正常对照组、高脂组、高脂维生素D3组和内皮损伤组,每组10只。分别以普通饲料、高脂饲料、高脂饲料+维生素D3负荷、高脂饲料+维生素D3负荷+内皮球囊损伤处理4个月,复制大鼠动脉粥样硬化形成过程中三个阶段的模型,即高脂血症、纤维增生性动脉硬化、较成熟的AS斑块病变。模型建造前,自颈动脉取血,模型建造成功后,自下腔静脉取血,用全自动生化仪测定血浆总胆固醇(TC)、总甘油三脂(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C),发色底物法测定血浆TAFI的活性,分析TAFI活性以及血脂指标在造模前、后不同组间的变化。结果:①成功复制了大鼠动脉粥样硬化发展过程中高脂血症期、纤维增生性动脉硬化期、较成熟的斑块期的模型;②高脂组、高脂维生素D3组和内皮损伤组大鼠的TC、TG、LDL-C水平和TAFI活性明显高于正常对照组(P<0.01),且有递增趋势,以内膜损伤组升高最为明显;三组模型鼠的HDL-C水平明显低于正常对照组(P<0.01或P<0.05),且有递减趋势,以内膜损伤组降低最为明显;③血浆TAFI活性与TC和TG水平呈正相关。结论:AS过程中,TAFI水平呈逐渐增高趋势,提示AS的病变程度与血浆TAFI水平密切相关。
Objective： To investigate the level of TAFI and its mechanism on atherosclerosis. Methods： Forty healthy male Wistar rats were randomly divided into 4 groups （n = 10）： the control group, the high lipid group, the high lipid ＋ Vitamin D overload group, and the endothelium injury group. Then the content of TC, TG, HDL-C, and LDL-C and the activity of TAFI were determined by a ehromogenie assay. Results： Atheroselerosis was successfully established. Hyperlipoidemia stage, fiber hyperplasy arteriosclerosis stage and maturatly plaque stage were also success- fully duplicated. TC, TG, LDL-C and the activity of TAFI in the plasma of the three model groups gradually increased compared with the control group （ P 〈0.01）. However, HDL-C in the plasma of the three model groups gradually decreased compared with the control group（ P 〈0.01 or P 〈0.05）. The activity of TAFI in the plasma positively related to TG and TC. Conclusion： The activity of TAFI in the plasma is closely correlated with atheroselerosis severity.
出处
《山东大学学报（医学版）》
CAS
北大核心
2007年第8期787-791,共5页
Journal of Shandong University:Health Sciences
基金
山东省科技发展计划项目(2006GG2202045)
