摘要
目的观察硫酸软骨素(ChS)对HL60细胞增殖的影响,探讨其对肿瘤生长的作用及其在恶性肿瘤化疗中的应用价值。方法应用细胞计数法进行细胞计数;用MTT法测定细胞存活率;用酶联免疫检测仪测定各组细胞的酸性磷酸酶活性。结果硫酸软骨素<50mg/L时显著促进HL60细胞增殖(P<0·01),但>50mg/L时无影响;加入阿霉素(ADR)后,ChS组的细胞生存率均有不同程度的降低,ChS浓度>25mg/L时明显降低(P<0·01);加入ChS后各组细胞的酸性磷酸酶活性明显增强,75mg/L时最为显著。结论硫酸软骨素在适当浓度时对HL60细胞的增殖有促进作用;ChS可增强ADR对HL60细胞的杀伤作用。
Objective To determine the proliferative activity of HL60 cells by ehondroitin sulfate （ChS）. Methods The proliferative activity was measured using cell counting after incubation with ChS of different concentrations. The acid phosphatase activity of each group was examined with ELISA to observe the differentiation of HL-60 cell. Results The concentration of ChS（ 〈 50 mg/L） increased the cell density of HL60 obviously （ P 〈 0. 01 ）, but the density of cell showed a little difference when the concentration increased above 50 mg/L. After ADR incubation, the cell surviving rate of each ChS group decreased in different degree, especially in the group of the ChS concentration above 25 mg/L （ P 〈 0. 01 ）. The acid phosphatase activity of each group increased very obviously, especially in 75 mg,/L group. Conclusion ChS with a proper concentration can accelerate the proliferative activity of HL60 cells ; It can increase the sensibility of HI.60 cell which added ADR and increase the killing effect of ADR obviously to HL60 cell.
出处
《基础医学与临床》
CSCD
北大核心
2007年第8期876-880,共5页
Basic and Clinical Medicine
基金
青岛市科技局科技计划项目(Kzd-15)
关键词
硫酸软骨素
增殖分化
细胞增殖
HL60
阿霉素
chondroitin sulfate
proliferation and differentiation
cell proliferation
HL60
ADR
