摘要
目的从眼镜蛇毒组分CⅡ中寻找抗肿瘤有效组分并研究其对多药耐药K562/A02细胞的杀伤作用。方法用SephadexG-75、SephadexG-25层析法从蛇毒中分离、纯化抗肿瘤有效组分,经SDS-PAGE电泳、HPLC分析、鉴定,用MTT法观察细胞毒性作用。结果CⅡ-3-1(近似电泳纯)的LD50为(0.622±0.007)mg/kg。CⅡ-3-1对耐药K562/A02及敏感K562细胞均有抑制作用。结论中华眼镜蛇毒中组分CⅡ-3-1对K562及K562/A02细胞都有显著杀伤作用。
Objective To discover the antineoplastic component C Ⅱ-3-1 of Naja naja atra （Chinese cobra） Venom and observe its effect on Multidrug-resistant human leukemia cell line K562/A02. Methods We isolate and purify C Ⅱ-3-1 from chinese cobra venom by G- 75 and G-25 MS chromatography assays, analyze and identify by SDS electrophoresis and HPLC assays, study its toxic action in human leukemia cells by MTT assays. Results The LD50 of Component CⅡ-3-1 was （0. 622±0. 007）mg/kg. The rate of K562 and K562/ A02 cell growth was obviously inhibited by Component C Ⅱ-3-1; Conclusion The rate of K562 and K562/A02 cell growth was obviously inhibited by Component C Ⅱ-3-1.
出处
《蛇志》
2006年第3期173-177,共5页
Journal of Snake
基金
广州市科技局资助项目(穗科条字[2005]6号)。
关键词
眼镜蛇
眼镜蛇毒
多药耐药
人白血病细胞
抗肿瘤药
Key words： Naja naja atra
Cobra venom
multidrug resistance
human leukemia cells
an- tineoplastic agents
