摘要
将分别采用普通酚-氯仿抽提法和试剂盒(柱吸附法)提取DNA的两种方法加以比较,以确定最适提取鱼糜制品DNA的方法。将鱼肉和鱼糜制品作为原料,用两种方法提取基因组DNA,利用分光光度计测定其A260与A280的吸光值,计算比率估计核酸的纯度,并利用琼脂糖凝胶电泳观察DNA片段在凝胶中的位置;并用线粒体16S rRNA基因PCR扩增产物鉴定所提取的基因组DNA。
The method of phenol-chloroform extraction and Kit （column） was compared to determine the optimal method to extract DNA from surimi products. The muscle of fish and surimi products would be as raw materials. It was inspected under spectrophotometer to get absorption value at 260nm and 280nm and estimated the rate of nucleic acid purity,observed the size of DNA fragments in agarose gel electrophoresis; identified the genomic DNA extracted using mitochondrial 16S rRNA gene PCR products.
出处
《湖北农业科学》
北大核心
2007年第4期520-522,共3页
Hubei Agricultural Sciences
基金
上海市重点学科建设项目(T1102)
