摘要
用免疫组织化学法研究了新生大鼠脊神经节与心肌细胞联合培养中降钙素基因相关肽的合成.在脊神经节与心肌细胞联合培养72小时的标本中,降钙素基因相关肽免疫反应呈阴性.在联合培养96小时的标本中,出现了许多降钙素基因相关肽免疫反应阳性神经纤维:表明在体外联合培养96小时以后,神经递质的合成才发育完成.
The synthesis of calcitonin gene-related peptide (CGRP) was examined by immunohis-tochemical method in the co-cultures of spinal ganglia and cardiac myocytes. CGRP-immunoreac-tive nerve fibers were not observed in the neuromuscular co-cultures at 72 hours of culture age, but there were many CGRP-immunoreactive nerve fibers in the neuromuscular co-cultures at 96 hours of culture age. The results indicate that the synthesis of neurotransmitter is not maturated in the neuromuscular co-cultures until 96 hours of culture age.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1997年第2期133-135,共3页
Chinese Journal of Anatomy
