普伐他汀对高糖培养大鼠肾小球系膜细胞增殖和氧化应激的影响被引量：7

Effects of Pravastatin on the proliferation of rat glomerular mesangial cells and oxidative stress under high concentration of glucose

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摘要目的:探讨普伐他汀对高糖培养的大鼠肾小球系膜细胞增殖和氧化应激的影响。方法:体外培养大鼠肾小球系膜细胞株,分对照组、高糖组和普伐他汀组,采用CCK-8细胞计数法测定系膜细胞增殖,比色法检测细胞培养液中的超氧化物歧化酶(SOD)活性和谷胱甘肽(GSH)、丙二醛(MDA)含量的变化。结果:与对照组比较,高糖组出现系膜细胞增殖增加,上清液中SOD活性、GSH含量下降,MDA含量增加;与高糖组相比,普伐他汀组系膜细胞增殖减少,GSH含量、SOD活性上调,MDA含量下降。结论:普伐他汀能抑制高糖环境下的系膜细胞增殖,并显著减少高糖诱导的氧化应激水平。 Objective：To investigate the effects of Pravastatin on the proliferation of cultured rat mesangial cells and oxidative stress under high concentration of glucose. Methods：Rat mesangial cells （MCs） were cultured in three groups in vitro： control groups, high glucose concentration group and pravastatin grouop. Cellular proliferation were assessed by CCK-8, the activity of superoxide dismutase （SOD） and the levels of malondialdehyde （MDA）、glutathione （GSH） in supernatant were assayed using chromatometry. Results： Compared with control groups, the growth of cells, the level of MDA content increased while the activity of SOD and level of GSH content declined in high glucose group; compared with that in high glucose group,the growth of cells and the level of MDA declined while the activity of SOD and GSH increased in pravastatin grouop. Conclusion： Pravastatin can inbibit high glucose-induced proliferation of mesangial cells, and attenuated oxidative stress.

作者金洁娜郑景晨倪连松尹丽慧沈飞霞

机构地区温州医学院第一附属医院内分泌科温州医学院第一附属医院医科所

出处《温州医学院学报》 CAS 2007年第4期347-349,共3页 Journal of Wenzhou Medical College

基金温州市科技局科研基金资助项目(Y2004A009)。

关键词糖尿病肾病氧化性应激普伐他汀肾小球系膜细胞 diabetic nephropathy oxidative stress pravastatin mesangial cells

分类号 R587.1 [医药卫生—内分泌]

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