摘要
目的研究中国西南特有濒危药用植物金铁锁的遗传多样性。方法应用AFLP分子标记技术对具有代表性的7个金铁锁居群,共137个个体进行分析。结果在金铁锁的物种水平上,Nei’s基因多样性指数(He)、Shan-non’s信息指数(I)、多态位点百分率(PPB)分别为0.2434±0.1791、0.3735±0.2485、82.30%;在其居群水平上分别为0.0918±0.1610、0.1402±0.2362、30.48%。居群间Nei遗传分化指数(Gst):0.6244;Shannon’s多样性基因遗传分化系数(Ist):0.6246。聚类表明:地理距离相对远的丽江居群与个旧居群遗传距离最近;昆明居群与其他居群遗传距离较远。统计获得9条可区分各地方居群的特征指纹带。结论金铁锁种内的遗传多样性水平丰富,而居群内遗传多样性水平较低,居群间遗传分化显著;各居群间亲缘关系与地理距离无明显相关性;其种内特征带与各居群特征带结合,可以为AFLP分子标记技术用于其种质资源的鉴定、遗传育种提供重要的依据。
Objective To analyze the genetic diversity of Psammosilene tunicoides, an endangered and endemic medicinal plant, in southwest China. Methods The genetic diversity of seven representational populations of P. tunicoides including 137 individuals had been investigated by amplified fragment length polymorphisms （AFLP） maker technique. Results The genetic diversity had been revealed as follow： the Nei＇ s genetic diversity index （He）, Shannon＇ s information index （I）, percentage of polymorphic loci （PPB） were 0. 243 4±0. 179 1, 0. 373 5±0. 248 5, and 82.30%, respectively at the species level; and 0. 091 8±0. 161 0, 0. 140 2±0. 236 2, and 30.48%, respectively at population level. The genetic differentiation index （Gst） was 0. 624 6 and genetic differentiation coefficient by Shannon＇ s diversity （Ist） was 0. 624 6. The result of dendrogram of seven populations indicated that Lijiang and Gejiu of Yunnan popula- tions shared the maximum genetic identity, though they distributed in a relatively great geographical distance; Kunming population of Yunnan had the greater genetic distance from other populations. Nine characteristic fingerprint bands that can distinguish the different populations had been acquired. Conclusion The genetic diversity of P, tunicoides is relatively higher at the species levels, while lower within population levels, and a significant degree of genetic differentiation oceurrs among the populations. There is little relativity between the relationship of populations and geographical distance. The combination of characteristic fingerprint bands between intraspecies and populations provide important basis data for germplasm resources diagnostics and plant breeding by AFLP maker technique.
出处
《中草药》
CAS
CSCD
北大核心
2007年第7期1070-1074,共5页
Chinese Traditional and Herbal Drugs
基金
云南省中青年学术技术带头人后备人才项目(2004PY01-18)
关键词
金铁锁
种质资源
遗传多样性
AFLP
Psammosilene tunicoides W. C. Wu et C. Y. Wu
germplasm resources
genetic diversi-ty
amplified fragment length polymorphism （AFLP）
