摘要
目的:检测20例慢性肾功能衰竭（CRF）患者红细胞膜ATP酶活力（ATPase）及红细胞内相关离子浓度。方法：红细胞膜ATPase活性测定参照Shalev方法。红细胞内Na＋、Ca(2＋)浓度测定采用原子吸收火焰法。结果：CRF患者Na＋－K＋－AT－Pase、Ca(2＋)－ATPase、Mg(2＋)－ATPase活性均非常明显低于正常人（P＜0．01）；但红细胞内Na＋和Ca(2＋)浓度却明显高于正常人（P＜0．01，P＜0．05）。CRF患者血清肌酐水平与红细胞内Na＋浓度存在明显正相关；Na＋－K＋－ATPase与Na＋含量之间呈负相关。结论：提示红细胞Na＋－K＋－ATPase活力降低是CRF患者酶学改变的表现之一。
To determine the ATPase activity of erythrocytic membrane and intraerythrocyticionic concentrations in 20 patients with chronic renal failure (CRF). Methods: Erythrocyte membrane AT-Pase activites were determined as described by Sha1ev, and erythrocyte Na+ and Ca2+ concentrations weredetected by atomic absorption spectrophotometry. Results: The Na+-K+-ATPase, Ca2 t -ATPase, Mg2+ATPase activities were significantly lower in CRF patients than in normal individuals (P<O. O1 ). Na+,Ca2+ were significantly higher in CRF patients. CRF patients with higher cell Na+ had higher serum creati-nine, and there was a significant p0sitive correlation between serum creatinine and erythr0cyte Na+ c0ncen-tration (r= O. 4824)- There was a significant negative relation between Na+-K+-ATPase activity and ery-throcyte Na+ concentration (r=0. 6420). Conclusion: There is a significant decrease of erythrocyte Na+-K+-ATPase and Ca2+, Mg’+-ATPase activity, suggesting this a feature of the changes of enzymology inCRF patients.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1997年第1期62-64,共3页
Academic Journal of Second Military Medical University
