摘要
为探讨白细胞介素２（ＩＬ－２）激活脐血单个核细胞（ＡＣＢ）对白血病细胞株ＨＬ－６０和Ｋ５６２细胞的杀伤作用以及脐血被激活后能否保持其造血祖细胞生成活性（ＰＣＡ），采用３Ｈ－胸腺嘧啶核苷前标记释放法和半固体培养等方法对其进行了研究。结果：ＩＬ－２激活的脐血细胞具有明显的抗肿瘤活性，且不影响其ＰＣＡ。ＩＬ－２激活脐血细胞的最适条件为：（１）细胞浓度为１×１０６ｍｌ；（２）ＩＬ－２浓度为１０００Ｕ／ｍｌ；（３）效靶比为１００１；（４）体外培养７２小时。脐血经ＩＬ－２激活后免疫表型发生明显变化，产生肿瘤坏死因子（ＴＮＦα）及白细胞介素６（ＩＬ－６），且生成大颗粒淋巴细胞（ＬＧＬ）。ＬＧＬ与Ｋ５６２细胞作用后，后者呈凋亡特征。研究结果为临床应用ＡＣＢ治疗白血病提供了实验依据。
To investigate efficacy of interleukin 2 (IL 2) activated cord blood (ACB) on lysis of leukemic cells and its progenitor cell activity (PCA), we analysed the efficacy of IL 2 ACB on the lysis to the K562 (NK sensitive) and HL 60 (NK resistant) cell lines as well as the potential mechanisms with the 3H TdR release assays. Our major results showed that IL 2 ACB produced significant lysis to leukemic cells and its optimum conditions Were as follows: (1) concentration of cells:1×10 6/ml; (2) concentration of IL 2∶ 1 000U/ml; (3) ratio of effector/target: 100∶1 and (4) time of culture: 72 hrs. Cord blood, after 24 hrs activation with IL 2, could produce tumor necrosis factor α (TNF α) and IL 6 and their immunophenotype altered. After cultured for 72hrs with IL 2, it could generate the large granular lymphocyte (LGL). After contacting with K562 cell a phenominon of apoptosis was also observed. In conclusion, our study basis for the treatment of leukemia by ACB.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
1997年第5期229-232,共4页
Chinese Journal of Pediatrics
