摘要
目的:分析运动强度与端粒酶表达及心肌细胞受损的关系。方法:实验于2005-04/11在华中师范大学体育学院人体科学实验室完成。①雄性SD大鼠30只,随机分为3组,对照组,有氧训练组,过度训练组,每组10只。②对照组不加任何训练;有氧训练组大鼠不负重,每天游泳训练75min,每周训练5d,休息2d。过度训练组大鼠每天游泳训练180min,每周训练5d,休息2d。从第4周开始,大鼠尾部负重,重物为体质量的5%,第一天负重5min,以后每次增加10min,直至180min全程负重。总训练时间16周。16周后处死,于左心室处取材,常规脱水石蜡包埋,切片,行苏木精-伊红染色。端粒酶TERTmRNA的检测采用原位杂交法,TERTmRNA表达阳性被染为棕黄色。用Image-proplus5.2图像分析系统测定阳性单位的积分吸光度值。结果:进入结果分析22只,对照组9只,有氧训练组8只,过度训练组5只。①有氧训练组心肌肌纤维平行排列,体积增大,细胞核呈椭圆形,体积较对照组增大。过度训练组肌纤维肿胀,细胞核增大,核间隙增宽,局部出现细胞核溶解,呈空泡状,肌浆凝聚和炎性细胞的浸润。②有氧训练组和过度训练组积分吸光度值高于对照组(54.876±25.883,142.08±56.621,0,P<0.01);过度训练组积分吸光度高于有氧训练组(P<0.05)。结论:运动可以促进端粒酶的表达,并且随运动强度的增大而增多,适宜的运动有助于端粒酶修复损伤的心肌细胞;过度运动时,端粒酶不能完全修复损伤的心肌细胞,心脏发生病理性改变。
AIM： To analyze the relationship of exercise intensity with telomerase expressions and cardiac myocyte damage. METHODS： The experiment was carried out in the Laboratory of Sports and Human Science, Department of Physical Education, Huazhong Normal University from April to November 2005. ①Thirty male SD rats were randomly divided into three groups including control group, aerobic exercise group, and overload exercise group with 10 rats in each group. ②The control group did not do any exercise; the aerobic exercise group swam for 75 minutes every day without any load for 5 days a week; the ovedoad exercise group swam for 180 minutes every day for 5 days a week, and from the fourth week, the tail of the rats loaded with 5% body mass of its body for 5 minutes everyday, following by 10 minutes increase until loaded for the whole 180 minutes. After 16 weeks training, the rats were killed to sample from the left ventricle for routine dehydration, paraffin imbedding, section and H-E staining. TERT mRNA was detected by in situ hybridization, and buffy meant positive expression. The integral optical density value of positive unit was measured by Image-proplus 5.2 image analysis system. RESULTS： Totally 22 rats were involved in the result analysis including 9 in the control group, 8 in the aerobic exercise group and 5 in the ovedoad exercise group. ①ln the aerobic exercise group, the cardiac muscle fiber arranged parallelly, and the volume was increased; cellular nucleus was ellipse, which was larger than that of the control group. In the ovedoad exercise group, the cardiac muscle fiber was swelling, and the nucleus was increased with wide crevice; karyolysis was found in part, and showed in vacuolous with muscle plasma condense and inflammatory cell infiltration.②The values of integral optical density of TERT mRNA in the aerobic and ovedoad exercise groups were higher than the control group （54.876±25.883, 142.08±56.621, 0, P〈0.01）; the values of the overload exercise group were higher than the aerobic exercise group （P〈0.05）. CONCLUSION： Exercise could promote the expression of telomerase, which increases with the exercise intensity. Proper exercise could help telomerase repair the damaged cardiac myocytes, but telomerase could not completely repair the damage under ovedoad exercise, and the pathological changes appear in the heart.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第29期5709-5711,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
