摘要
本研究用反相蒸发技术制备出包裹IL-2 DNA或IL-6 DNA的脂质体,将其与体外培养的靶细胞共育,或将其直接注射至小鼠腹腔内及荷瘤小鼠的瘤体内,研究其介导体内外基因转移的效果,结果表明我们研制的脂质体不但能将目的基因转移至体外培养的靶细胞中,并可通过腹腔内注射及瘤体内注射后,在腹腔内及肿瘤原位直接将IL-2和IL-6基因转移至腹腔细胞及肿瘤细胞中,稳定表达IL-2和IL-6。
In the present study, we prepared cationic liposomes encapsulated IL-2 DNA or IL-6 DNA with reverse-phase evaporation to investigate their efficiency in mediating gene transfer. After transfection with IL-2 DNA liposome or IL-6 DNA liposome, the supernatants of NIH3T3 cell contained high levels of IL-2 or IL-6. The peritoneal cells from the mice injected i.p. of IL-2 DNA or IL-6 DNA liposome 10 days later could also be detected to secrete IL-2 or IL-6. The B16F10 melanoma cells from tumor-bearing mice which were injected intratumorally of IL-2 DNA or IL-6 DNA liposome exhibited resistant to G418 and secreted high levels of IL-2 or IL-6. The results show that the liposome can mediate cytokine gene transfer efficiently in vitro and in vivo.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
1995年第3期213-217,共5页
Chinese Journal of Cancer Biotherapy
基金
国家自然科学基金(39421009)的资助
