摘要
白细胞介素１１（Ｉｎｔｅｒｌｅｕｋｉｎ－１１，ＩＬ－１１）是一种具有广泛生物学功能的造血生长因子，体内体外实验均显示ＩＬ－１１具有显著的造血促进作用。为了探讨ＩＬ－１１基因治疗应用于造血功能低下疾病的可能性，构建了含人ＩＬ－１１ｃＤＮＡ的逆转录病毒载体，并转染小鼠成纤维细胞系ＮＩＨ－３Ｔ３，经筛选获得３株高表达ＩＬ－１１的转染细胞亚克隆。用高表达ＩＬ－１１的转染细胞作为载体细胞，进行了ＩＬ－１１基因治疗对小鼠造血系统影响的实验研究。结果表明ＩＬ－１１基因治疗能显著提高正常小鼠外周血血小板水平；明显缩短亚致死量放射损伤小鼠白细胞降低的时间，促进外周血白细胞、血小板和红细胞压积的恢复。提示ＩＬ－１１基因治疗对于血小板减少性疾病治疗和放射损伤后的造血恢复具有潜在的应用价值。
Interleukin 11 is a multifunctional cytokine,especially in hematopoiesis.Successfully transfected the hIL 11 cDNA into murine fibroblast cell line NIH 3T3 cells and obtained the high secreting subclone 1E7 for further genetherapy experiment.Results showed that IL 11 genetherapy had significant effects on both normal and sublethally irradiated mice including an increase in the peripheral platelets of normal mice and marked acceleration of the recovery of peripheral WBC and platelets in sublethally irradiated mice.The results suggested that IL 11 could play an important role in the regulation of hematopoiesis under physical condition and IL 11 genetherapy had a potential value in hematopoiesis recovery.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1997年第2期71-75,共5页
Chinese Journal of Immunology
