摘要
研究了成纤维细胞介导的人白细胞介素６（Ｉｎｔｅｔｒｌｅｕｋｉｎ－６，ＩＬ－６）基因疗法对预先体内注射１５０ｍｇ／ｋｇ５－氟尿嘧啶（５－ＦＵ）所造成的造血损伤小鼠模型的治疗作用。结果发现，外周血血小板数量在移殖高分泌ＩＬ－６成纤维细胞后第１０天开始持续上升，第２２天血小板数量恢复到化疗前水平，中性粒细胞数量也显著增高，但对白细胞恢复没有明显的促进作用。骨髓和脾脏ＣＦＵ－ＧＭ及ＣＦＵ－ＭＫ在体内移殖高分泌ＩＬ－６成纤维细胞后，体外动态观察发现：骨髓和脾脏ＣＦＵ－ＧＭ、ＣＦＵ－ＭＫ在第４～７天左右数量为０，在第１０天左右数量开始上升，于第１６天左右数量显著增高，对ＣＦＵ－ＧＭ、ＣＦＵ－ＭＫ具有明显的恢复作用，ＣＦＵ－Ｓ数量也显著增高。表明成纤维细胞介导的白细胞介素６基因疗法能显著治疗化疗导致的造血损伤，可望为肿瘤放、化疗导致的机体造血损伤提供新的治疗途径。
n the present study , we examined the effect of fibroblast mediated human interleukin-6(IL-6) gene therapy on the mice that have been injected intraperitoneally 5-fluorouracil(5-FU)at a dose of 150mg/kg. We observed that the number of plateletes in peripheral blood increasedat day 10 after implantation of IL-6 highly-secreting fibroblast cells and returned to normal atday 22. Neutrophil counts were also elevated , but WBCs were not accelerated to recover fromchemotherapy. In the colony formation, CFU-GM、CFU-MK in bone marrow and spleen was notfound at day 4～7 after implantation of IL-6 highly-secreting fibroblast cells. The numbers ofCFU-GM. CFU-MK were enhanced after day 10 and had a significant increase after day 16.CFU-GM、CFU-MK in bone marrow and spleen were accelerated to recover. CFU-S was alsomarkedly argumented in mice. These data demonstrated that fibroblast-mediated human IL-6gene therapy can treat chemotherapy-induced hematopoietic suppression effectively. The experi-ment outlines a new approach to treat chemotherapy or radiotherapyinduced hematopoietic sup-pression.
出处
《中国免疫学杂志》
CSCD
北大核心
1995年第3期153-157,共5页
Chinese Journal of Immunology
基金
国家自然科学基金和军队八五攻关基金
关键词
白细胞介素6
基因治疗
成纤维细胞
药物疗法
interleukin6 Gene therapy Fibroblast ChemotherapyHematopoietic recovery
