摘要
在pH5的乙酸盐缓冲介质中,甲基紫与透明质酸钠作用形成结合产物时将导致溶液共振瑞利散射（RRS）大大增强并产生新的RRS光谱,其最大散射峰位于341 nm处。透明质酸钠在0~2.5 mg·L^-1范围内其浓度与RRS强度成正比。该法具有高灵敏度,对透明质酸钠的检出限（3σ）为20.1μg·L^-1,选择性较好。此法用于测定从鸡冠组织中提取的透明质酸的粗品,测定结果的平均相对标准偏差为2.3%,加标平均回收率为98.9%。
In a buffer medium of pH 5. 0, a compound complex was formed between sodium hyaluornate （abbreviated as NaHA） and methyl violet, leading to a great enhancement of the intensity of resonance Rayleigh scattering （RRS） and giving a new RRS spectrum, with its maximum scattering peak at 341 nm. It was also found that the intensity of RRS was directly proportional to the concentration of NaHA in the range of 0-2. 5 mg · L^-1. Based on these facts, a sensitive method for the determination of NaHA was proposed. The detection limit （3a） of this method was found to be 20. 1μg · L^-1, Good selectivity of the method was shown by the results of interference test. The proposed method has been applied to the determination of NaHA in crude product of NaHA extracted from cockscomb, giving av. value of RSD＇s of the results of 2. 3% and av. recovery of 98. 9%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2007年第1期8-10,14,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
重庆市教委科研项目(NO.KJ051305)
关键词
共振瑞利散射
透明质酸钠
甲基紫
Resonance Rayleigh Scattering
Sodium hyaluronate
Methyl violet
