摘要
应用ＥＬＩＳＡ测定了５４例类风湿性关节炎患者、９９例非类风湿性关节炎患者以及１００例正常人的血清Ⅱ型胶原抗体，结果阳性率分别为９０．７％、０、０。５４例类风湿性关节炎患者中有７例血清类风湿因子为阴性而Ⅱ型胶原抗体为阳性，且病程均在半年之内。结果表明：Ⅱ型胶原抗体的检测对类风湿性关节炎具有特异性诊断和早期诊断的临床意义。
Type Ⅱ collagen was prepared from the articular carlilage of human fresh dead fetus.This collagen had been comfirmed by amino acid analysis and SDS-PAGE.An improved enzymelinked immunosorbent assay(ELISA)for the detection of anticollagen antibodies in human serum had been developed.With the use of this method,antibodies against native human type Ⅱcollagen were detected in 90.7% of sera from 54 patients with rheumatoid arthritis(RA).The antibodies were all negative in sera from 99 patients with non-RA diseases and 100 normal human. Thus anti-type Ⅱcollagen antibody assay may have specific diagnostic significance for RA patients.
