摘要
以清洁级ICR雄性小白鼠为实验动物，研究不同剂量乙酸铜对小白鼠的生殖毒性。采用小白鼠精子畸形实验及小白鼠骨髓嗜多染红细胞（以下简称PCE）微核实验等方法。分别对成年小白鼠腹腔每天注射0．25～16．00mg／kg 8个剂量的乙酸铜，染毒7天。结果表明：乙酸铜对小白鼠的体重增长及睾丸重量具有一定的抑制作用，其中组Ⅵ、组Ⅶ的最明显。不同剂量的乙酸铜均使雄性小白鼠精子密度P〈0．001）、精子活力明显降低，具有明显的剂量效应。各实验组精子畸形率、PCE微核率均明显高于对照组（P〈O．001）（P〈0．05或P〈0．001），且均随乙酸铜剂量的增加而明显升高。结果表明实验剂量的乙酸铜对ICR雄性小白鼠具有明显的生殖毒性效应。
The sperm deformity test and PCE micronucleus test were used to study the different doses of copper acetic acid on reproduction toxicity of male mice. The copper acetic acid of 0.25-16.00 mg/kg/d were respectively investigated by intraperitoneal injection to male mouse for 7 days. The results show that the copper acetic acid in each experimental group can inhibit the increases of body weight and testis, of which group VI and VII are the most remarkable. They can also reduce significantly the vitality and concentration of sperm. There is an apparent dose-effect. Besides, the frequencies of sperm deformity and micronucleus in each experimental group are significantly higher than the control group＇s. Moreover, they rise with increasing dose of copper acetic acid. The results show that experimental doses of copper acetic acid have obvious effects of reproduction toxicity on ICR male mice.
出处
《细胞生物学杂志》
CAS
CSCD
2007年第3期439-443,共5页
Chinese Journal of Cell Biology
基金
温州市科技局科研基金(No.S2002A015)
温州师范学院科研基金(No.2003Z20)资助项目
关键词
乙酸铜
生殖毒性
精子活力
精子畸形率
微核率
copper acetic acid
reproductive toxicity
the vitality of sperm
the frequency of spermdeformity
the frequency of micronucleus
