摘要
目的:探讨外源性胰岛素对离体大鼠胰岛第一时相胰岛素分泌的影响.方法:雄性SD大鼠胰腺采用Ⅴ型胶原酶经胰管灌注消化分离得到胰岛,用100μU/mL外源性胰岛素分别孵育0,60,120和240min(n=8)后,给予葡萄糖刺激,分别测定0,3,5,10和30min胰岛素分泌量.结果:不同时间胰岛素预处理后,各组在葡萄糖刺激时均产生胰岛素第一时相分泌(P<0.05),但随着胰岛素孵育时间的延长,胰岛素第一时相分泌峰值降低(P<0.01).结论:100μU/mL外源性胰岛素损害胰岛素第一时相分泌,且孵育时间越长,抑制程度越明显.
AIM： To investigate the effect of exogenous insulin on first-phase insulin secretion of rat islets in vitro. METHODS ： Male SD rat islets were isolated using digestion by ductal injection of collagenase V and incubated with exogenous insulin （ 100 μU/ mL） for 0, 60, 120 and 240 min （n = 8）respectively. After incubation with insulin, the islets were stimulated by glucose （ 16.7 mmol/L）. The secretion amount of insulin was measured at 0, 3, 5, 10 and 30 min, respectively. RESULTS： The first-phase glucose-stimulated insulin release existed in all groups （P 〈 0.05 ）. But the peak of first-phase insulin release decreased as the incubation time of exogenous insulin went by （ P 〈 0. 01 ）. CONCLUSION： The first-phase glucose-induced insulin secre- tion is impaired by exogenous insulin pretreatment.
出处
《第四军医大学学报》
北大核心
2007年第12期1101-1103,共3页
Journal of the Fourth Military Medical University
关键词
胰岛
细胞
培养的
大鼠
胰岛素
时间反应曲线
islets of langerhans
cells, cultured
rats
insulin
time response curve
