摘要
目的:探讨新生鼠胰岛的分离和纯化,为体外培养胰岛和胰岛细胞奠定基础。方法:将100只新生Wistar大鼠分为10组,每组10只,用V型胶原酶消化胰腺,用质量分数35%牛血清白蛋白(BSA)和葡聚糖作为纯化剂,分离和纯化胰岛。结果:每组胰岛收获量为(780±23)个,纯度达92%,活性为86%,胰岛的分泌功能良好。结论:联合应用35%BSA和葡聚糖分离纯化新生鼠胰岛是可行的。
Objective： To explore the separation and purification of pancreatic islets in neonatal rats. Methods： One hundred neonatal Wistar rats were randomly divided into 10 groups. The pancreas were digested by collagenase V, islets were purified by combination of dextran and 35% bovine serum albumin（BSA）. Results： The mean islet yield of each goup was 780 ±23, purity was 92%, viability was 86%, and the islets had good biological activity. Conclusion： The use of dextran combined with 35 % BSA is a feasible method for isolation of islets in neonatal rats.
出处
《汕头大学医学院学报》
2005年第3期178-179,F0004,共3页
Journal of Shantou University Medical College
