摘要
目的:探讨p75NTR在面神经损伤后面神经核运动神经元再生中的作用。方法:对p75基因敲除小鼠和野生型小鼠的面神经总干进行损伤,损伤后第4天,在面神经总干损伤的远中侧神经分叉处切断,将切断的面神经断端置入含有FastBlue的聚乙烯单端小管中进行后示踪标记,术后第7天冷冻切片后荧光显微镜下对面神经核运动神经元进行计数,然后进行统计分析。结果:两组之间再生的神经元数量有显著性差异(P<0.05)。结论:p75NTR可以促进面神经运动神经元的再生。
Objective： To investigate the role of p75NTR in the regeneration of facial nerve nuclei during nerve crush injury. Methods： At the 4th day after injury of facial nerve, nerve trunk was cut in the bifurcated region and the stump was inserted into a small polymer tube containing Fast blue. Retrograde tracing and labelling motoneuron counting were used to examine the survival of motoneurons in the facial nerve nuclei in p75 knock-out mice and wild type mice respectively. Results： Retrograde tracing showed that the number of survival motoneurons from the p75 knock-out mice was much fewer than those from the wild type mice（P〈0.05）. Conclusion： p75NTR can promote the survival rate of motoneurons in the facial nerve nuclei after nerve injuries.
出处
《口腔颌面外科杂志》
CAS
2007年第2期139-142,共4页
Journal of Oral and Maxillofacial Surgery
