摘要
目的通过不同浓度的羟基磷灰石(HAP)纳米粒子作用于肝癌H22小鼠肿瘤细胞,研究其对肿瘤细胞凋亡的影响。方法100只肝癌H22小鼠随即分组并连续服用不同剂量的HAP纳米粒子,11d后处死小鼠,计算抑瘤率和细胞凋亡率,评价不同剂量的HAP纳米粒子与H22小鼠肿瘤细胞生长的关系。结果肝癌H22小鼠肿瘤细胞对纳米羟基磷灰石(HAP)较敏感,能明显延长小鼠生存期,高剂量组与丝裂霉素结果相近(P>0.05)。实验后小鼠体重没有明显下降,抑瘤率和细胞的凋亡率随浓度升高而增大,呈明显的剂量依赖性(P<0.01)。结论不同浓度的羟基磷灰石(HAP)纳米粒子能够抑制H22肿瘤细胞的增殖,诱导肿瘤细胞的凋亡,并呈剂量依赖性。
Objective To explore the effects of different dosage of hydroxyapatite ceramic （HAP） Nano-particles on apoptosis of H22 tumor cell line in mice hepatic cancer. Methods 100 mice with tumor of H22 were divided into groups randomly, which taken different dosage of HPA Nano-particles in different group one time a day. After 11 days, all the mice were sacrificed and the inhibition rate and the cell apoptosisowere calculated to evaluate the relationship between dosage of HAP Nano-particles and tumor growth. Results Tumor of H22 were sensitive to HAP Nano-particles, and could also prolong the life span of mice （P〈0. 01）. The results was approximate to that of mitomycin group （P〉0.05）o The weight of the mice did not decrease. The rate of apoptosis and that of growth inhibiting increased with the rising concentration of the HAP Nano-particles. Conclusion HAP Nano-particles can induce apoptosis of the tumor H22 cell line in mice, which is dose-dependent.
出处
《解剖学研究》
CAS
2007年第3期168-170,共3页
Anatomy Research
