摘要
目的观察不同浓度的羟基磷灰石纳米粒子对肝癌SMMC-7721细胞作用的影响。方法将羟基磷灰石纳米粒子以不同浓度和时间作用于肝癌SMMC-7721细胞,采用MTT比色法观察细胞毒性;应用电镜技术及HE染色法观察凋亡细胞的形态;原位末端标记法(TUNEL法)检测细胞凋亡率。结果羟基磷灰石纳米粒子以剂量依赖和时间依赖的方式抑制SMMC-7721细胞的生长,作用48h后的半数有效抑制浓度IC50值为179.8μg/ml。HE染色和透射电镜观察到凋亡特性的形态学改变。结论HAP抑制SMMC-7721细胞的增殖,诱导细胞凋亡,HAP也许会成为临床治疗肝癌的有效抗肿瘤药物。
Objective To investigate the effects of different concentrations of hydroxyapatite （HAP） nanoparticles on human hepatocellular carcinoma cell line SMMC-7721. Methods SMMC-7721 was treated with HAP nanopartricles at various concentrations for different duration to detect cellular toxicity by Methabenzthiazuron （MTT） method, and to observe apoptosis-related alterations in morphology with Hematoxylin and eosin （HE） staining and transmission electron microscopy （TEM） and to detect apoptosis by TUNEI. Results HAP nanoparticles inhibited the growth of SMMC-7721 in a dose and time dependent manner, with IC50 values of 179. 8μg/ml 48 h after treatment. Under HE staining and TEM, the cells treated with HAP nanoparticles exhibited characteristics of apoptosis. Conclusions HAP nanoparticles not only inhibit the proliferation but also induce apoptosis of human hepatocellular carcinoma, which provide the evidence in the treatment of hepatocellular carcinoma, and might be an effective anti-tumor drug.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2006年第1期79-81,共3页
Chinese Journal of Gerontology
基金
黑龙江省教育厅科研基金资助(10541218)
关键词
羟基磷灰石纳米粒子
细胞凋亡
肝癌细胞
Hydroxyapatite nanoparticles
Apoptosis
Hepatocellular carcinoma
