摘要
目的：比较慢性内脏痛觉敏化模型的两种行为学评价指标,即腹壁撤退反射和腹外斜肌放电测量,为内脏痛觉敏化提供较为客观、有效的评价方法。方法：SD大鼠出生后8～15天内每天给予一次结直肠扩张（CRD）刺激以后正常饲养至第八周,分别采用腹壁撤退反射（AWR）评分法和腹外斜肌放电测量方法评估大鼠受CRD刺激时的痛觉敏化程度。结果：AWR评分方法比较模型组与对照组,在20～60 mmHg CRD时评分模型组显著高于对照组,在80 mmHg CRD差异不明显;对照组痛阈为30.42±1.34 mmHg,模型组痛阈为13.13±1.34 mmHg,（P〈0.001）;腹外斜肌放电测量,在20～60 mmHg CRD时模型组放电明显高于对照组,80 mmHg CRD差异不明显;两组痛阈分别为对照组37.5±4.53 mmHg,模型组25.0±3.27 mmHg,（P〈0.05）。结论：两种方法均可作为慢性内脏痛觉敏化模型是否成功的筛选指标,相比之下,前者较为简便,后者较为客观。
Objective ： To establish an objective and effective method for the assessment of a model of visceral hypersensitivity by comparing abdominal withdrawal reflex （AWR） with spike of external oblique muscle of abdomen （EOMA）. Methods： The model of visceral hypersensitivity was established by colorectal distensions （CRD） on neonatal rats beginning 8 - 15 days after their birth. After the rats were kept to eighth week, responses to CRD were compared by AWR or spike of EOMA. Results： Measurement of the scores of AWR between model and control rats demonstrated significant difference, when CRD at pressures of 20 -60 mmHg, but no significant difference occurred at 80 mmHg. The pain threshold was 30.42 ±1.34 mmHg in control rats and 13.13± 1.34 mmHg in model rats （P 〈0. 001 ）. Measurement of the amplitudes of spike of EOMA showed significant difference, the CRD at pressures of 20 - 60 mmHg, while no significant difference occured at 80 mmHg. The pain threshold was 37.5 ±4.53 mmHg in control rats and 25.0 ± 3.27 mmHg in model rats （ P 〈 0.05 ）. Conclusion ： Both behavioral indexes can be used to a model of chronic visceral hypersensitivity. The former is more convenient, while the latter is more objective.
出处
《中国疼痛医学杂志》
CAS
CSCD
北大核心
2007年第3期153-156,共4页
Chinese Journal of Pain Medicine
基金
福建省自然科学基金(项目编号:C0510011)
福建省科技厅基金(项目编号:FJGxy04009)资助
关键词
SD大鼠
内脏高敏
痛阈
肌电
腹壁撤退反射
Sprague-Dawley rats
Visceral hypersensitivity
Pain threshold
Myoelectricity
Abdominal withdrawal reflex
