摘要
目的 应用束缚应激大鼠实验模型 ,研究离体结肠平滑肌的收缩运动及其影响因素 ,探讨鸟苷素在结肠运动中的作用。方法 建立束缚应激大鼠动物模型 ,制备离体结肠平滑肌环行肌及纵行肌肌条 ,应用张力换能器 ,测定其肌张力。应用放射配基法测定结肠组织及血浆中鸟苷素含量。结果 束缚应激刺激可诱发大鼠排便增加 ,该动物模型是较好的模拟人IBS的实验动物模型。束缚应激大鼠离体结肠平滑肌的张力升高 ,对K+、Ca2 +、乙酰胆碱作用的反应明显加强 ,其血浆及结肠组织中鸟苷素的含量增加 ,曲美布汀可增强基础状态结肠平滑肌的收缩 ,降低氯化钾、氯化钙及乙酰胆碱对结肠的收缩作用。结论 结肠平滑肌本身收缩活动的异常是肠易激综合征发病的重要机制之一 ,调节平滑肌收缩与舒张运动 ,纠正肠管平滑肌运动异常 ,是治疗肠易激综合征的新方向。
Objective To investigate the influence of contraction of isolated colonic smooth muscle and role of guanylin in colonic movement in wrap restraint stress rats. Methods By using wrap restraint stress induced defecation rats, the tone of isolated longitudinal and circular colonic muscle strips and guanylin contents in colon and blood were measured by transducer and radioligand binding assay respectively. Results There were similarities between the intestinal effects of wrap restraint stress in rats and irritable bowel syndrome (IBS) in human . Therefore, wrap restraint stress rat is an appropriate animal model to study stress related intestinal dysfunction. In wrap restraint stress rats, both the tone of smooth muscle and the reaction to K +,Ca 2+ and acetycholine increased significantly; meanwhile guanylin contents in blood and colon increased also. Trimebutine can decrease the basic tone of smooth muscle and reaction to Ca 2+ 、 K +and acetycholine. Conclusions The abnormality of colonic smooth muscle contraction is one of the mechanisms in IBS and regulation of colonic smooth muscle contraction is a new method to treat IBS.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2000年第9期588-591,共4页
Chinese Journal of Internal Medicine
