摘要
以根癌土壤杆菌(Agrobacterium tumefaciens)WSHAT12为出发菌株,通过硫酸二乙酯诱变,获得遗传稳定性好的抗L-乙硫氨酸(Eth)突变株WSH-E01,通过进一步的诱变处理,获得L-乙硫氨酸和维生素K3(VK3)双抗性突变株WSH-V01,以双抗性突变株WSH-V01为出发菌株,再进行诱变处理,获得一株X-gal利用能力提高的突变株WSH-X01,与出发菌株WSHAT12相比,突变株WSH-X01的辅酶Q10产量提高幅度达50.6%,同时,对突变株WSH-E01的发酵条件进行优化。出发菌株WSHAT12、突变株WSH-E01、WSH-V01和WSH-X01在优化后的发酵条件下辅酶Q10产量分别达到23.1mg/L、26.8mg/L、29.5mg/L和34.8mg/L。
Agrobacterium tumefaciens WSH AT12 was mutagenized by using DES and screened with ethionine. A mutant WSH-E01 was obtained after two runs of screening. WSH-E01 was further treated with DES and screened with VK3, and a new mutant WSH-V01 （Eth^rVK3^r） was obtained. WSH-V01 was mutagenized again and a mutant WSH-X01 （Eth^rVK3^r X-gal^b） was obtained. The fermentation conditions of WSH-E01 were investigated in shaking flasks. Under the optimized fermentation conditions, the CoQl0 yield of WSH AT12, WSH-E01, WSH-V01 and WSH-X01 reached 23. lmg/L, 26.8 mg/L, 29.5mg/L and 34.8mg/L, respectively.
出处
《工业微生物》
CAS
CSCD
北大核心
2007年第3期5-9,共5页
Industrial Microbiology
关键词
根癌土壤杆菌
诱变
辅酶Q10
优化
Agrobacterium tumefaciens
mutagenesis
CoQl0
optimization
