摘要
目的:探讨DNA甲基转移酶抑制剂5-杂氮-2′-脱氧胞苷(5-aza-CdR)对人食管癌细胞株Eca109增殖作用的影响。方法:应用不同浓度(10-7、10-6、10-5、10-4mol/L)的特异性DNA甲基转移酶抑制剂5-aza-CdR,于不同作用时间(24～168h)处理人食管鳞癌细胞株Eca109,采用四甲基偶氮唑蓝(MTT)法测定5-aza-CdR作用下Eca109细胞株的生存率,倒置显微镜观察细胞的形态学变化。结果:不同药物浓度组在同一作用时间下,细胞株生存率差异有统计学意义(P<0.05);同一药物浓度不同作用时间组之间(24～96h)的细胞生存率差异也有统计学意义(P<0.05),且96h时对人食管鳞癌细胞株Eca109生长抑制作用最明显。形态学观察显示:癌细胞体积缩小,核固缩,染色质凝聚成块。结论:5-aza-CdR有抑制人食管癌细胞株Eca109生长的作用,抑制作用呈时间依赖性及浓度依赖性;并且干预后癌细胞呈现典型的凋亡细胞形态改变。
Objective： To explore the effect of the DNA methyltransferase-5-aza-CdR on human esophageal squamous cell cancer Eta109 line. Methods.. Human esophageal squamous cell cancer Eta109 line were treated by 5-aza-CdR with 10^-7, 10^-6,10^-5 ,10^-4 mol/L, respectively. Consequently, the growth rate of the cells was detected by MTT assay and morphological pattern observation. Results： The proliferation of Eta109 cells was inhibited by 5-aza-CdR from 10^-7 to 10^-4 mol/L. Moreover, the inhibition rate show time- and-concentration-dependent manner （24-96 h, 10^-7-10^-4 mol/L, P 〈0.05）, inhibitory peaks were achieved peak at 96 hours in each concentration groups and apoptotic morphology was observed by invert- microscope. Conclusion. The proliferation of Eta109 cells was inhibited by 5-aza-CdR in a time-and-concentration-dependent manner （24-96 h, 10^-7-10^-4 mol/L） through apoptosis pathway.
出处
《新疆医科大学学报》
CAS
2007年第4期349-351,共3页
Journal of Xinjiang Medical University
基金
中国博士后科学基金(20060390535)
新疆维吾尔自治区高技术研究发展计划基金(XJKJT20051113)
乌鲁木齐市科学技术厅基金(Y05331002)
新疆维吾尔自治区高校科研计划青年教师科研基金(XJEDU2004S14)
