摘要
应用聚丙烯酰胺凝胶垂直板电泳（ＰＡＧＥ）分离和研究成体泥鳅心肌、骨骼肌、肝脏、卵巢、脑、眼、血清七种组织器官中的ＬＤＨ同工酶。结果表明：在电泳图谱负极端的相同部位，骨骼肌出现５条，肝脏有４条，脑组织１条ＬＤＨ同工酶区带；其余多数组织中均为３条酶区带；此外，近负极端，肝脏组织还有１条其他组织所没有的ＬＤＨ同工酶区带。这提示了泥鳅的ＬＤＨ同工酶可能是由ＬｄｈＡ、ＬｄｈＢ和ＬｄｈＣ３个基因座位编码的Ａ、Ｂ、Ｃ亚基组合成的四聚体。定量分析表明ＬＤＨ同工酶活力具有明显的组织特异性：血清、卵巢、肝脏等组织中ＬＤＨ５活性最强。
The electrophoretic patterns of LDH isoenzymes in serum, eye, brain,ovary,liver, skeletal muscle and cardiac muscle from adult M. anguillicaudatus distributed over bog were studied by means of polyacrylamide gel vertical electropheresis. The results indicated that in the same area near negative pole there were three bands in the most of these tissues, but one band in brain, four bands in liver and five bands in skeletal muscle respectively.In addition, in the different area near positive pole there was one band in liver. All these suggest that the LDH isoenzymes of M. anguillicaudatus might be tetraner made up by A, B and C subunit codied by three gene loci,namely Ldh-A, Ldh-B and Ldh-C. The quantitative analysis exhibited a tissue specific expression: the strongest activity of the LDH isoenzyme were LDH 5 in serum, ovary and liver, LDH 2 in cardiac muscle and LDH 3 in skeletal muscles.
出处
《安徽农业大学学报》
CAS
CSCD
1997年第1期68-72,共5页
Journal of Anhui Agricultural University
关键词
泥鳅
LDH同功酶
电泳图谱
.anguillicaudatus LDH isoenzyme tetraner
