摘要
背景与目的肿瘤周围血管的生成以及细胞黏附功能的变化是肿瘤具有恶性潜能的重要特征。在众多影响肿瘤侵袭和转移的因素中,整合素因其特殊的结构和功能日益受到重视。本研究的目的是探讨整合素β1 mRNA、β3 mRNA在肺鳞癌中的表达及其与肿瘤微血管密度(MVD)及生物学行为的关系。方法应用组织芯片、原位杂交、免疫组化等方法,检测152例肺鳞癌中整合素β1mRNA、β3 mRNA和CD34的表达。结果整合素β1 mRNA高表达组与低表达组间MVD值比较,差异无统计学意义(P=0.072)。整合素β3 mRNA高表达组与低表达组的MVD值比较,差异有统计学意义(P=0.041)。β1 mRNA、β3 mRNA高表达率与淋巴结转移(P=0.018,P=0.011)、远处转移(P=0.020,P=0.035)和T分期(P=0.025,P=0.015)有密切关系。β1mRNA、β3 mRNA高表达组的生存期显著短于低表达组(P=0.034,P=0.021)。MVD≥39的患者生存期显著短于MVD<39的患者(P=0.024)。结论整合素β1和整合素β3均参与肺鳞癌的侵袭和转移过程,并与预后相关。整合素β3与肺鳞癌血管的生成相关。整合素β1可能与肺鳞癌的早期发生有关。整合素β1、β3的表达可能作为判定肺鳞癌血管生成、生物学行为及评估预后的有意义的指标。
Background and objective It has been proven that the newborn vessels and adhesion ability are very important characters of malignant tumor cells. Among all the invasion and metastasis factors, integrins seem to be hot spots gradually because of their special construction and function. The aim of this study is to investigate the expression of integrin β1 mRNA and integrin β3 mRNA in squamous cell carcinoma （SCC） of the lung, as well as their correlation with microvascular density （MVD） and biological behaviour of SCC. Methods Expression of integrin β1 mRNA, β3 mRNA and CD34 protein was detected in 152 SCC specimens by tissue microarray, in situ hybridization and immunohistochemistry. Results The mean MVD was not significantly different between high and low integrin β1 mRNA expression groups （P= 0. 072）, however, it was markedly different between high and low integrin β3 mRNA expression groups （P= 0. 041）. The high expression rate of integrin β1 mRNA and β3 mRNA was closely related to lymphatic metastasis （P=0. 018, P= 0.011）, distant metastasis （P=0.020, P=0.035） andTstage（P=0.025, P=0.015）. The survival time of patients with high integrin β1 mRNA and β3 mRNA expression was significantly shorter than that of those with low expression （P=0. 034, P= 0. 021）. The survival time of patients with MVD value≥39 was significantly shorter than that of those with MVD value〈39 （P=0. 024）. Conclusion The results of this study suggest that both of integrin β1 and β3 may be involved in invasion and metastasis of SCC, and that integrin β3 expression correlates with enhanced tumor angiogenesis and integrin β1 expression may be an early event in development of SCC. They may serve as prognostic markers of SCC.
出处
《中国肺癌杂志》
CAS
2007年第2期128-132,共5页
Chinese Journal of Lung Cancer
