摘要
研究了两个居群的金耳Tremella aurantialba以及近似品的rDNA ITS区碱基全序列的特征及其差异，首次报道了金耳的ITS和5．8SrDNA完整序列，序列总长度为467～468，长度变异较少。聚类分析表明两个居群金耳亲缘关系非常密切，金耳药材和近似品金黄银耳形成一个稳定的独立分支，近似品黄金银耳形成另一个分支。ITS序列的差异为金耳的鉴别提供了可靠的分子标记，为金耳菌类药材基原入药建立了遗传基础。
The rDNA ITS sequences from two populations in China were studied and three analog species were discussed together. The whole sequences of rDNA ITS regions （ including ITS-1,5.8S and ITS-2） of Tremella aurantialba were reported first time. The results showed that little difference in length between the sequences was found and the length was 467bp and 468bp,respectively. Tremella aurantialba and Tremella aurantia formed a separate stable branch in the topology trees,while Tremella mesenterica clustered on another clade. The relationship between two populations of Tremella aurantialba in Yunnan and Shanxi provinces and Tremella aurantla is closely related,and they can replace each other as the same medicine according to the rDNA ITS. The character of ITS sequences can be used for providing the molecular markers for identifying Tremella aurantialba and exploring its genetic basis of fungal sources.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第2期216-220,共5页
Natural Product Research and Development
关键词
金耳
核糖体DNA
ITS序列
居群
近似品
DNA分子鉴别
Tremella aurantialba
rDNA
ITS sequence
population
analog species
DNA molecular identification
