摘要
对我国四个地区牡丹主要栽培品种rDNA ITS区序列进行测定,研究各居群rDNA ITS序列特征及差异,建立不同地区主要牡丹栽培品种的区域性分子标记,参照GeneBank信息(登录号:U27692)利用Clustal X、MEGA3.1分子进化遗传分析软件对测序结果排序。牡丹ITS全序列长度为652 bp,相对于GenBank中牡丹rD-NA ITS全序列在448位少一个C碱基,其中ITS1为267 bp,5.8 S为163 bp,ITS2为222 bp,GC含量为55.7%～56.9%,共有30个变异位点,主要发生在ITS1、ITS2区,5.8S区也存在多个位点的变异。牡丹rDNA ITS区序列特征(SNPs)可用于不同地区主要牡丹栽培品种的鉴别。
The coding regions of rDNA ITS gene in different groups of peonies were sequenced to study phylogenetie relationships of 19 Paeonia species. According to the characteristics and variation of ITS gene sequence of Paeonia from different areas, the molecular markers of peonies were proposed by eonsiderlng the discordance between various taxa. The rDNA ITS regions were compared with the GeneBank information （accession No. U27692） , analyzed and identified the polymorphism （SNP） sites in the different groups of peonies by Clustal X,MEGA 3.1. The length of rDNA ITS gene sequence of Paeonia varieties was 652 bp,ineluding ITS1 （267 bp） ,5.8S（ 163 bp） and ITS1 （222 bp）. The GC content was about 55.7%-56.9%. Compared with the rDNA ITS geue sequences of Paeonia （U27692）, the specific 448^th site in the samples identified to be C in database was lost. There were some coding SNPs loci in the conservative region of 5.8S as well as about 30 kinds of SNPs in non-coding region ITS1 and ITS2. By ITS gene-sequenee analysis, Paeonia cultured in Chongqing can be distinguished from those referred in this study.
出处
《天然产物研究与开发》
CAS
CSCD
2009年第2期225-230,266,共7页
Natural Product Research and Development
基金
重庆市自然科学基金(7351)
