摘要
目的研究高胆固醇饮食对AD大鼠模型认知功能损害和海马齿状回神经元损伤的影响。方法海马齿状回注射β淀粉样蛋白(Aβ)建立阿尔茨海默病大鼠模型,根据不同饮食,将动物分为高胆固醇+Aβ组、高胆固醇+BPS组、标准饮食+Aβ组和标准饮食+BPS组;采用morris水迷宫检测大鼠定向导航和空间探索能力,应用尼氏染色方法检测海马齿状回神经元数量的变化。结果高胆固醇饮食加重AD大鼠认知功能的损害,平均逃避潜伏期长于其余各组。与其他组相比,高胆固醇饮食Aβ组的穿环数以及平台象限游泳距离所占百分比减少;高胆固醇饮食Aβ组海马齿状回针道附近神经元数目(23±2.2)显著少于标准饮食Aβ组(54±5.1)、高胆固醇饮食磷酸盐缓冲液(BPS)组(86±8.2)和标准饮食BPS组(90±8.6)。结论高胆固醇饮食加重AD大鼠模型海马齿状回神经元损伤,进而加剧AD大鼠认知功能损害。
Objective To study the effect of high cholesterol diet on the cognition performance and neuronal loss of AD rats. Method AD rat model was established by local injection of the amyloid-β peptide （Aβ1-40） in PBS vehicle into the right hippocampus. The rats were divided by type of diet into four groups, cholesterol（CH）＋ Aβ group, standard diet（SD）＋ Aβ group, cholesterol （CH） ＋ BPS group, standard diet（SD）＋ BPS group. Learning and memory ability were determined by Morris water maze. Neuronal loss was evaluated by means of Nissel staining. Results CH＋AD rats exhibited seriously spatial learning and memory det＇icits in both place navigation trial and spatial probe trial. In place navigation trial, the mean value of escape latency of Ch＋Aβ group was longer than that of the other groups. In spatial probe trial,Ch＋Aβ group had shorter distance percentage of spending in the former platform quadrant and fewer frequency of crossing the former platform site than the other group. The result also revealed the greater neuronal loss in the vicinity of pin hole was found in CH＋AD rats（23±2.2） than in SD＋AD rats（54±5.1） ,Ch＋BPS rats（86±8.2）and SD＋BPS rats（90±8.6）. Conclusion High cholesterol diet facilitates neuronal loss in dentate gyrus of AD rat model, which in turn aggravates cognition impairment of AD rats .
出处
《重庆医学》
CAS
CSCD
2007年第9期849-851,共3页
Chongqing medicine
关键词
胆固醇
饮食
阿尔茨海默病
认知功能
cholesterol
diet
Alzheimer＇ s disease
cognition performance
