摘要
以兰州百合为研究对象,通过鳞片离体培养、抗生素筛选以及离子束处理等,建立了稳定高效的基因转化受体系统。结果表明:MS+6-BA1.0mg/L+NAA0.5mg/L是不定芽诱导的适宜培养基,1/2MS+NAA0.2mg/L+0.1%活性炭是较为适宜的生根壮苗培养基。抗生素敏感性试验表明,卡那霉素和头孢霉素选择压分别以125mg/L和300mg/L较为适宜。运用能量为30keV的低能氮离子束注入百合鳞片,发现8×10^(14)N+/cm^2的剂量对不定芽的诱导具有明显的刺激和促进作用。
The scale leaf of Lily was taken as explant. Though the experiments of the in vitro culture of Lily, the sensibility of adventitious buds to several antibiotic and the treatment of ions implantation, a stable and efficient acceptor system of gene transformation of Lanzhou Lily was established. The results showed that the medium of MS＋ 6-BA 1.0mg/L＋NAA 0. 5mg/L was optimum for the differentiation and elongation of adventitious buds. The 1/2MS＋ NAA0. 2rag/L＋0. 1%C was the suitable medium for adventitious root. The findings of experimems of the sensibility of antibiotic showed that the selection concentration of katmamycin and cefotasimine are 125mg/L and 300mg/L respectively. By the implantation of 14N ion beams with the energy of 30keV, preliminary results showed that the induction of adventitious buds was greatly stimulated at the dosage of 8×10^14 ions/cm^2.
出处
《北方园艺》
CAS
北大核心
2007年第4期215-217,共3页
Northern Horticulture
基金
国家自然科学基金(30470105)
关键词
兰州百合
组织培养
基因转化
离子注入
Lanzhou Lily
Tissue culture
Gene transformation
Ion implantation
