摘要
将能量为６０ｋｅＶ、剂量为２×１０１６Ｎ＋／ｃｍ２的氮离子束分别注入３个玉米自交系的成熟种子．从其Ｍ１成株上取下未成熟的胚作外植体，在ＭＮ和ＡＧ培养基上进行高体培养，以未注入的相同材料为对照．结果发现，Ｍ１的平均愈伤组织诱导频率比对照高１１．７％，其中在ＭＮ和ＡＧ培养基上，分别比对照高１５．５％和７．９％、不同类型的材料，在两种培养基上的愈伤组织诱导频率存在差异，而且在诱导过程中Ｍ１与对照材料均要求６－ＢＡ的浓度在０．５－１．５ｍｇ／Ｌ。
Immature embryos from three self-bred series of M1 corn(Zea mays)were cultured in MN and AG medium after dry seeds were implanted to 2×1016cm-2 N+ ions of 60keV and grown into M1 plants. The experiments indicated that all genotypes formed pale-yellow or yellow callus in the two media. The average callus induction frequency of M1 immature embryos was 11.7% higher than that of the control. Percentages of callus were different not only for various genotypes of the culture material in the two media, but also for different concentration of 2,4-D and 6-BA.
出处
《核技术》
CAS
CSCD
北大核心
1995年第7期410-414,共5页
Nuclear Techniques
基金
"国家"八五"重点攻关项目
辽宁省科委重点研究项目
关键词
玉米
细胞培养
氮离子束
Corn(Zea mays),Nitrogen ions implantation,Callus inductivity,Plant regeneration
