摘要
利用前列腺组织特异性的基因载体新型Ｃ３（１）和转基因技术，培植出人ｂｃｌ２基因的前列腺组织特异性转基因鼠。免疫组织化学检测证实人ｂｃｌ２蛋白在鼠前列腺上皮细胞中的过量表达。病理学观察发现，转基因鼠已发生良性前列腺增生。该实验为说明ｂｃｌ２基因在良性前列腺增生（ＢＰＨ）中的病理作用提供了最直接的证据，并繁殖出可稳定传代的ＢＰＨ的动物模型。
To establish an animal model for prostate hyperplasia,three lines of transgenic mice were generated by using the prostate specific rat C3(1) promotor to drive expression of the human bcl 2 cDNA. The tissue specific overexpression of human bcl 2 protein in prostatic epithilium was confirmed by immunohistochemical analysis.Male transgenic mice develop atypical benign prostate hyperplasia by 10 months of age in up to 50% of animals. The experiment provides the immediate evidence of the critical role of protooncogene bcl 2 in the development of BPH.Moreover, these transgenic mice offered an suitable animal system to study the etiology of BPH.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1997年第2期97-99,共3页
Chinese Journal of Urology
