摘要
采用抗ｂｃｌ－２单克隆抗体对２０例前列腺增生（ＢＰＨ）和１２例正常前列腺组织的冰冻切片进行免疫组织化学染色。结果表明，正常前列腺的外周区ｂｃｌ－２蛋白主要位于腺体的基底细胞，腺上皮细胞偶见着色，移行区仅部分基底细胞阳性着色。前列腺增生组织中的上皮基底细胞和大部分腔上皮细胞均呈强阳性，阳性的细胞数和染色强度均高于正常前列腺组织。具有抑制细胞凋亡的ｂｃｌ－２蛋白在ＢＰＨ组织中的过表达提示ＢＰＨ的发生可能与上皮细胞可以不依赖于激素的生长和（或）细胞寿命延长，凋亡的细胞数目减少有关。
Bcl-2 oncoprotein is known to protect cells from apoptosis.The aim of this study was to investigate whether bcl-2 correlates with the development of benign prostatic hyperplasia(BPH).Expression of bcl-2 was investigated in 20 cases of BPH and in 12 cases of normal prostate tissue using immunohistochemical technique.All basal cells and some luminal cells of peripheral zone in normal prostate were positive.In central and transtional zone,bcl-2 expression was limited to some dispersed basal cells.In BPH,bcl-2 was strongly expressed in basal cells and most of the luminal cells.The rate of positive cells and its staining intensity were much higher in BPH than in normal prostate.There was no bcl-2 experession in the mesenchyme.The results suggest that high expression of bcl-2 might have a role in the development of BPH.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1995年第12期729-731,共3页
Chinese Journal of Urology
关键词
前列腺增生
免疫组织化学
癌蛋白
Benign prostatic hyperplasia Immunohistochemistry
