摘要
目的:利用脉冲场凝胶电泳(PFGE)技术探讨O139群霍乱弧菌的分子分型方法。方法:用SfiⅠ酶和NotⅠ酶切O139群霍乱弧菌染色体DNA,经脉冲场凝胶电泳进行片段分离。结果:14株O139群霍乱弧菌经SfiⅠ酶切后电泳分离可得一个PFGE型,用NotⅠ酶切电泳可分为2个PFGE型。结论:脉冲场凝胶电泳技术分析O139群霍乱弧菌,分型发现微小差别有助于分析追踪疫情和来源。
Objectlve：To study the gene typing methods of Vibrio cholerae O139 by pulsed -field gel electrophoresis（PFGE）, Methods：The chromosome DNA of Vibrio cholerae O139 was digested by Sfi Ⅰ enzyme and Not Ⅰ enzyme and the PFGE was carried out. Results： There was 1 genotype by Sfi Ⅰ enzyme and 2 genotypes by Not Ⅰ enzyme in 14 strains of Vibrio cholerae O139. Conclusion：The fine typing ability and reliability of PFGE are helpful for analyzing the epidemic situation and finding out the infectious resource of the outbreak.
出处
《中国卫生检验杂志》
CAS
2007年第4期622-623,共2页
Chinese Journal of Health Laboratory Technology
