摘要
细菌中散在分布的DNA重复序列近年来不断被报道,基因外重复回文序列和肠细菌基因间共有重复序列是两个典型的原核细胞基因组散在重复序列。重复序列在染色体上的分布和拷贝数具种间特异性,用它们的互补序列作为引物,以细菌基因组DNA为模板进行PCR扩增反应,反应产物的琼脂糖电泳可以提供非常清晰的DNA指纹图谱,使用此图谱既可对各种微生物进行快速分型及鉴定,又可对它们进行DNA水平上的遗传多样性分析。细菌基因组重复序列PCR技术具有简捷、快速、结果稳定等特点,可对细菌进行分子标记,用于菌株分型、分类鉴定和亲缘关系等方面的研究。
Dispersed repetitive DNA sequences in bacteria have been continuously reported recently. Repetitive Extragenic Palindromic （REP） elements and Electro-Bacterial Repetitive Intergenic Consensus （ERIC） sequences are two synthesized prokaryotic repetitive elements. Distribution of the elements in chromosome and copies possesses interspecific specificity, using their complementary feature as a primer and genotypic DNA of bacteria as a template to carry out PCR amplification reaction, the agarose gel electrophoresis of the reaction products could provide dear DNA fingerprint for rapid analyzing different bacterial species and strains, and could carry out diversity analysis of their genetic. Bacteria repetitive-element PCR technique is a simple, fast, stable in result, and can be used as molecule marker to study molecular classification, identification, and phylogenetic relationship of strains and species.
出处
《微生物学杂志》
CAS
CSCD
2006年第3期90-93,共4页
Journal of Microbiology
关键词
细菌
重复序列PCR
分类鉴定
bacteria
repetitive-element PCR
classification and identification
