摘要
探讨重组人白细胞介素６（ＩＬ－６）对人肺癌生长调控作用及机制。方法以人肺巨细胞癌系ＰＧ和人肺腺癌细胞系ＰＡａ为研究对象，研究ｒｈＩＬ－６对ＰＧ、ＰＡａ细胞体外生长的作用；并应用Ｎｏｒｔｈｅｒｎ杂交检测ｒｈＩＬ－６对ＩＬ－６、ＩＬ－６受体（ＩＬ－６Ｒ）、ｃ－ｍｙｃ、ｃ－ｆｏｓ、ｃ－ｊｕｎ等基因表达的影响。结果发现ｒｈＩＬ－６促进ＰＧ、ＰＡａ细胞生长，呈浓度依赖性和时间依赖性。逆转录聚合酶链反应检测证实，ＰＧ、ＰＡａ细胞均表达ＩＬ－６及ＩＬ－６ＲｍＲＮＡ。ｒｈＩＬ－６上调ＩＬ－６、ＩＬ－６Ｒ及ｃ－ｍｙｃ等基因表达，而对ｃ－ｆｏｓ、ｃ－ｊｕｎ基因表达无明显影响。结论ｒｈＩＬ－６促进ＰＧ、ＰＡａ细胞生长；ｒｈＩＬ－６上调ＩＬ－６、ＩＬ－６Ｒ及ｃ－ｍｙｃ等基因表达作用可能与其促进ＰＧ。
Objectives To detect the activity of interleukin 6(rhIL 6)on human lung cancer and the influences of recombinant human IL 6(rhIL 6)on the in vitro growth of high metastatic human lung giant cell carcinoma cell line PG and low metastatic human lung adenocarcinoma cell line PAa.Methods The effects of rhIL 6 on the expressions of IL 6,IL 6R,c myc,c fos and c jun mRNAs were analyzed by Northern blotting hybridization.Results rhIL 6 could stimulate the in vitro growth of both PG and PAa cells,in adose and time dependent manner.Both PG and PAa cells were showen to express IL 6 and IL 6Ridentified by reverse transcription PCR analysis.Northern blotting hybridization revealed that rhIL 6up regulated mRNA levels of IL 6,IL 6R and c myc rather than those of c fos and c jun.Conclusion These data suggest that rhIL 6may promote the proliferation of human lung cancer cell lines PG and PAa,which might,at least partly,be related to the up regulation of expressions of IL 6,IL 6 R,c myc gene transcript.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第3期194-196,共3页
National Medical Journal of China
