摘要
利用人肺巨细胞癌株PLA801-95D细胞悬液,在通气的旋转摇动培养系统中形成球体。在体外,直径0.2mm的PLA801-95D球体与圆形预培养的鸡胚心肌块(PHF)接触培养,表现出很强的侵袭力和很高的恶性程度。在体内,直径0.3mm的PLA801-95D球体分别移植于裸小鼠耳廓皮下和背部皮下,结果耳廓皮下移植裸小鼠未见肿瘤生长,背部皮下移植5只裸小鼠中3只在接种局部出现侵袭性生长,并有引流淋巴结和肺转移。提示PLA801-95D细胞株在体内外恶性程度均较高。
Invasion modols of tumor spheroid aggregates in vitro and in vivo were established by using human giant cell carcinoma (lung strain PLA801-95D). PLA801-95D cells from monolayer cultures were suspended and allowed to form spheroid aggregates for 3 d on a gyratory shaker. In organ culture, PLA801-95D(0.2mm in diameter) were confronted with 0.4mm round precultured heart fragments (PHF), then co-cultured on a gyratory shaker. PLA801-95D cells showed a strong capacity of invasion in vitro.In vivo, PLA801-95D spheroids (0.3mm in diameter) were transplanted subcutaneously into the auricles and flanks of BALB/c nude mice. No s. c. tumor growth was noted in the auricle 4 mon hs after transplantation, but at the s. c. flank, :umors were found in 3 of 5 mice, wi.h invasive growth seen at ths site of transplatation. Metastases were found in regional drainage lymph nodes (axillary, submaxillary and inguinal lymph nodes) and in the lungs.PLA801-95D cells had high malignancy in vitro and in vivo. Both spheroid invasion models proved to be suitable for judging the malignancy of human tumor Cells and for studying the mechanism of human tumor invasion.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1991年第5期353-358,共6页
Acta Academiae Medicinae Sinicae
基金
七五"攻关课题
关键词
肺肿瘤
瘤细胞球体
侵袭
转移
human giant carcinoma tumor spheroid aggregates invasion metastasis
