摘要
研究了球孢白僵菌在不同浓度、施菌方式、温度和相对湿度下对温室蚜虫的侵染力以及接种后对蚜虫的侵染速率。结果表明,高(109个孢子.mL-1)、中(108个孢子.mL-1)、低(107个孢子.mL-1)3个浓度剂量对蚜虫都有较强的致病力,且浓度越大,蚜虫的死亡率越高,死亡时间越提前。用浸渍法和孢子浴法接种蚜虫,6 d的累积死亡率分别为100%和31.1%。在测试的3个温度(22、26、30℃)中,26℃时蚜虫侵染力最强,第3天出现死亡高峰,第5天时累积死亡率就达到100%,明显高于22、30℃的处理。相对湿度越大,球孢白僵菌的致病力越强,蚜虫死亡速度越快。在温湿度组合中相对湿度为95%时,温度对白僵菌的侵染力几乎无影响,但影响发病速度,相对湿度低于95%时,26℃的侵染力始终高于22和30℃时的侵染力。通过接种后不同时间段用0.2%百菌清处理蚜虫测定该菌株的侵染速率,结果表明接种后24 h是该球孢白僵菌有效侵染蚜虫的关键时期。
The virulence of Beauveria bassiana against Myzus persicae was bioassayed under different inoculation concentration, inoculation methods, temperature, and relative humidity. The infection rate after inoculation was also examined. The result showed that with three concentrations of 10^9, 10^8 and 10^7 mL^-1 of B. bassiana tested was highly virulent to aphids. Moreover the more the conidia exposed to aphids, the higher the cumulative mortality of aphids was and the earlier aphids died. When the aphids were soaked in the conidia suspension and exposed to spore shower of B. bassiana the cumulative mortality after inoculating 6 days was 100% and 31.1% respectively. The aphids had higher mortality rate at 26 ℃ than that of at 22 and 30 ℃. The death apex occurred on the 3rd and the cumulative mortality was 100% on the 5th at 26 ℃. Among the regions of 22 -30 ℃ with 95% RH, the temperature had little effect on the cumulative mortality of aphids but greatly affected the development of diseased. With lower 95% RH, the virulence of B. basslana was always higher than that of at 22 and 30 ℃. In order to measure the infection rate of B. bassiana, the conidial attached on the surface of aphids was disinfected using 0.2% chlorothalonil solution at different intervals after inoculation. The result indicated that the first 24 h after inoculation was crucial to the infectin of B. bassiana to M. persicae.
出处
《华东昆虫学报》
2006年第4期253-257,共5页
Entomological Journal of East China
基金
辽宁省"十五"科技攻关项目(2006215004)
辽宁省工程技术研究中心专项(200535)
沈阳农业大学青年基金项目
关键词
球孢白僵菌
蚜虫
侵染
Beauveria bassiana
Myzus persicae
infection
