摘要
目的分析磷酸化AKT(p-AKT)及其下游分子Cyclin D1、MMP-9在非小细胞肺癌(NSCLC)中的表达情况,探讨p-AKT及其下游分子在肺癌发病机制中的作用。方法应用免疫组化的方法,检测80例NSCLC组织及35例非肿瘤性肺组织标本中p-AKT、Cyclin D1和MMP-9的表达,并与临床病理因素进行相关性分析。结果p-AKT、Cyclin D1和MMP-9在NSCLC中高表达,阳性率分别为78.8%、76.3%和72.5%,显著高于非肿瘤性肺组织中阳性表达0、0和11.4%(P<0.05)。p-AKT在高中分化腺癌中阳性表达(95.0%)显著高于低分化腺癌的表达(50.0%)(P<0.05),而与患者年龄、性别、组织学类型及鳞癌的分化程度、TNM分期、淋巴结转移无关(P>0.05)。CyclinD1、MMP-9表达与淋巴结转移、鳞癌的分化程度有关(P<0.05),MMP-9还与TNM分期有关(P<0.05),p-AKT的表达与Cyclin D1呈正相关(rs=0.787,P<0.01),与MMP-9蛋白表达无关(rs=0.022,P>0.05)。结论在NSCLC中存在AKT的活化,Cyclin D1的高表达可能与AKT的活化有关,MMP-9可能通过其他的调控机制参与了肺癌的发生发展。在NSCLC组织中存在着AKT信号转导通路的激活。
[Objective] To investigate the expression of phospho-AKT （p-AKT） and its target gene products including Cyelin D1 and MMP-9 in human non-small cell lung cancer （NSCLC） tissue, and to explore the mechanisms in tumorgenesis of NSCLC. [Methods] The expression of p-AKT, Cyelin D1, MMP-9 in 80 cases of NSCLC and 35 cases of no-cancerous lung disease were assessed by immunohistoehemistry, and their correlations with eliniopathological factors were statistically analyzed. [Results] The positive rate of p-AKT, Cyelin D1, MMP-9 was 78.8%, 76.3%, 72.5% in NSCLC and 0%, 0%, 11.4% （P 〈0.05） in no-cancerous lung disease. The positive rate of p-AKT in Well-differentiated and moderately-differentiated adenoeareinoma （95.0%） was significantly higher than that of p- AKT in poorly-differentiated adenoeareinoma （50.0%） （P 〈0.05）. The expression of p-AKT didn＇t relate to age, sex, histologie subtype and Squamous cancer differentiation, lymph node metastasis and TNM stages （P 〉0.05）. The expression of Cyelin D1 and MMP-9 correlated with lymph node metastasis and differentiation of squamous cell carcinoma （P 〈0.05）, The expression of MMP-9 correlated with TNM stages （P 〈0.05）. In addition, The expression of p- AKT had a positive correlation with the expression of Cyelin D1 （P 〈0.01） and no correlation with the expression of MMP-9 （P 〉0.05）. [Conclusion] AKT activation may be present in NSCLC tissues. The high positive rate of CyclinD1 may correlate to activation of AKT. MMP-9 may participate in carcinogenesis and development of lung cancer through other routes. PI3K/AKT signaling pathway is activated in NSCLC tissues.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2007年第7期786-790,共5页
China Journal of Modern Medicine
基金
国家自然科学基金项目(No.30571552)
河南省教育厅科技攻关项目(No.2006320049)
