摘要
目的:探讨磷酸化蛋白激酶B(P-AKT)在非小细胞肺癌(NSCLC)中的表达及其与肺癌血管生成的关系。方法:应用免疫组织化学S-P法检测P-AKT、血管内皮细胞生长因子(VEGF)以及微血管密度(MVD)在46例NSCLC组织中的表达,并分析三者的表达与NSCLC临床病理特征的关系以及三者之间的相关性。结果:NSCLC中P-AKT、VEGF的阳性率分别为43.5%和67.4%,明显高于正常肺组织的0%和22.2%(P<0.05)。P-AKT的表达与肺癌的分化程度、分期、淋巴结转移密切相关(P<0.05)。VEGF的表达与肺癌的分期、淋巴结转移密切相关(P<0.05)。肺癌、正常肺组织中MVD值分别为24.62±6.21和13.26±5.96,两者相比,差异有统计学意义(P<0.05)。MVD值的高低与肺癌的分期、转移、分化程度密切相关(P<0.05)。P-AKT在肺癌中的表达与VEGF和MVD值密切相关(P<0.05)。结论:P-AKT在肺癌组织中的表达显著增高,并与肺癌的分期、转移和分化相关,P-AKT可以通过促进肺癌血管的生成导致肺癌的转移。
Objective： To investigate the levels of microvessel density（MVD）, the expression of P-AKT and vascular endothelial growth factor（VEGF） in non-small cell lung cancer （NSCLC）, and explore the correlation between them and the clinicopathological features of NSCLC. Methods： MVD and expressions of P-AKT and VEGF in 46 specimens of NSCLCS tissues and 18 specimens of normal lung tissues were detected by SP immunohistochemistry. Their interrelations and correlations were analyzed. Results： Positive rate of P-AKT and VEGF were significantly higher in NSCLC than in normal lung tissues （43.5% vs 0% and 67.4% vs 22.2%, P 〈 0.05） ; there were correlations between P-AKT expression and lymph node metastasis, between the TNM stage and differentiation of NSCLC （ P 〈 0.05） and between VEGF expression and lymph node metastasis and the TNM stage of NSCLC（ P 〈 0.05）. The MVD of lung cancer tissues was higher than that of normal lung tissues（24.62 ±6.21 vs13.26± 5.96, P 〈 0.05） ; there were correlations between the MVD and lymph node metastasis and between the TNM stage and differentiation of NSCLC （ P 〈 0.05）. There were positive correlations between P-AKT expression and VEGF expression and MVD（ P 〈0.05）. Conclusion： P-AKT is over-expressed in NSCLC, which correlates with lymph node metastasis, the TNM stage and differentiation of NSCLC and takes part in the angiogenesis of NSCLC.
出处
《山东大学学报（医学版）》
CAS
北大核心
2007年第2期184-187,191,共5页
Journal of Shandong University:Health Sciences
关键词
癌
非小细胞肺
蛋白激酶类
血管内皮细胞生长因子
微血管密度
Carcinoma, non-small-cell lung
Protein kinases
Vascular endothelial growth factor
Microvessel density
