摘要
目的探讨苦参碱对胰腺癌细胞SW1990生长抑制和诱导凋亡作用。方法将不同浓度的苦参碱作用于培养的SW1990细胞,通过MTT比色法、流式细胞仪和DNA凝胶电泳、电子显微镜技术观察检测细胞凋亡,研究分析其对SW1990细胞的生长抑制和诱导凋亡作用。结果苦参碱浓度为0.5、1.0、1.5g/L时,细胞生长抑制率分别为14.3%、24.8%、41.6%;流式细胞术测定RD横纹肌肉瘤细胞的凋亡率分别为10.3%、18.3%、27.5%;DNA电泳见DNA“梯形”图谱;电镜显示肿瘤细胞的凋亡形态。结论苦参碱能抑制SW1990细胞增殖,诱导其凋亡。
Objective To study the growth - inhibition and the mechanism of apoptosis induced by matrine on pancreatic cancer cells. Methods Pancreatic cancer cells SW1990 were treated with matrine for 3 days. The cytotoxicity was observed by MTT assay. The apoptotic rates were shown by flow cytometry（FCM）. Electron microscopy and DNA gel electrophoresis were applied to demonstrate the presence of apoptosis of SW1990. Results Pancreatic cancer cells treated with matrine （0. 5, 1.0,1.5g/L）, the cytotoxicity index were 14. 3% ,24. 8% ,41.6% ,respectively, the apoptostic rates were 10. 3% ,18.3% ,27.5% ;The obvious apotosis morphological changes in pancreatic cancer cells were shown by electron microscope. Conclusion Matrine is able to inhibite the proliteration and induced the apoptosis of Pancreatic cancer cells SW1990.
出处
《医药论坛杂志》
2007年第5期20-21,共2页
Journal of Medical Forum
关键词
横纹肌肉瘤
苦参碱
凋亡
Pancreatic cancer
Matrine
Apoptosis
