摘要
目的探讨苦参碱对人RD横纹肌肉瘤细胞生长抑制和诱导凋亡作用。方法将不同浓度的苦参碱作用于培养的RD横纹肌肉瘤细胞，通过MTT比色法、流式细胞仪和DNA凝胶电泳、电子显微镜技术观察检测细胞凋亡，研究分析其对RD横纹肌肉瘤细胞的生长抑制和诱导凋亡作用。结果苦参碱浓度为0．5、1．0、1．5g／L时，细胞生长抑制率分别为13．2％、23．7％、40．5％；流式细胞术测定RD横纹肌肉瘤细胞的凋亡率分别为10．1％、17、7％、26．3％；DNA电泳见DNA“梯形”图谱；电镜显示肿瘤细胞的凋亡形态。结论苦参碱能抑制RD横纹肌肉瘤细胞增殖，诱导其凋亡。
Objective To study the growth - inhibition and the mechanism of apoptosis induced by matrine on RD rhabdomyosarcoma cells. Methods Rhabdomyosarcoma cells were treated with matrine for 3 days. The cytotoxicity was observed by MTT assay. The apoptotic rates were shown by flow cytometry （FCM）. Electron microscopy and DNA gel electrophoresis were applied to demonstrate the presence of apoptosis. Results Rhabdomyosarcoma cells were treated with matrine （0.5,1.0,1.5 g/L） ,the cytotoxicity index were 13.2%, 23.7% ,40.5%, respectively, the apoptostic rates were 10.1%, 17.7% ,26.3%. The obvious apotosis morphological changes in rhabdomyosarcoma cells were shown by electron microscope. Conclusion Matrine is able to inhibit the proliteration and induce the apoptosis of rhabdomyosarcoma cells.
出处
《中原医刊》
2007年第8期25-26,共2页
Central Plains Medical Journal
关键词
横纹肌肉瘤
苦参碱
凋亡
Rhabdomyosarcoma
Matrine
Apoptosis
