摘要
目的:探讨雷公藤内酯醇(TL)对人胰腺癌细胞SW1990移植瘤的生长及新生血管生成的抑制作用。方法:通过人胰腺癌裸鼠皮下移植实验,观察不同剂量TL对移植瘤生长抑制作用;应用免疫组化和RT-PCR研究裸鼠移植瘤组织VEGF表达变化,计算肿瘤组织微血管密度(MVD)。结果:各实验组(TL0.125、0.25和0.5mg·kg-1·day-1)抑瘤率分别达到66.16%、78.14%和89.92%,与对照组相比,肿瘤生长明显受抑,并具有剂量和时间依赖性。对照组和各实验组瘤组织MVD分别为36.25±8.64、22.75±6.67、17.65±7.11和9.87±3.34(P<0.01);TL抑制移植瘤VEGF基因和蛋白表达,且VEGF基因下调与MVD的减少具有相关性(r=0.7424,P<0.01)。结论:TL具有显著的抗胰腺癌移植瘤作用,其机制可能与抑制肿瘤新生血管生成有关。
To explore the inhibitory effects of Triptolide （TL） on tile growth and anglogenesis of transplanted human pancreatic tumors in nude mice. Methods： Different dosages of TL were administered to nude mice that were injected with SW1990 cells. The inhibitory effects on the growth of tumor xenografts and tumor microvascular density （MVD） were investigated. VEGF protein and mRNA expression were detected by immunohistoehemistry and semi-quantitative RT-PCR. Results： TL significantly suppressed the growth of tumor when administered at 0.125, 0.25 anti 0.5 mg/kg·day, and the inhibitory rate was 66.16%, 78.14% and 89.92%, respectively. The control group showed the highest MVD level of 36.25±8.64, while the 0.5 mg/kg.day TL group displayed the lowest level of 9.87±3.34. TL caused a dose-and time-dependent inhibitory effect on VEGF protein and mRNA expression. A positive correlation was found between VECF expression and MVD in the tumors （r=0.742 4, P〈0.01）. Conclusion： The inhibitory effects of TL on transplanted human pancreatic tumor cells in nude mice may partially be due to its antiangiogenie activity. TL should be examined further as a novel anti-neoplastic agent for the treatment of pancreatic tumors.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2007年第19期1121-1125,共5页
Chinese Journal of Clinical Oncology
基金
江苏省自然科学基金资助(编号:BK2004049)
