摘要
对“海沃德”与“皖翠”RAPD扩增的2条分子量为740bp、649bp的特异带,进行了分离、纯化、克隆与测序,根据测序结果设计出4条20bp的特异引物,进行SCAR-PCR扩增。SCAR-PCR扩增结果显示,分子量为649bp的特异带在“皖翠”与“海沃德”中都扩增出了条带,说明“皖翠”这一RAPD扩增特异带并不存在;“皖翠”扩增出现了分子量为740bp的特异带,而“海沃德”未出现此带,证明“海沃德”与“皖翠”存在此变异位点,成功地将“皖翠”变异的RAPD标记转化为SCAR标记,此标记可应用于“皖翠”品种的快速鉴定。
Two special bands,with the molecular size of 740 bp and 649 bp between Actinidia deliciosa cv. Hayward and Wancui wereseparated, purified, cloned and sequenced. Two couple of special primers in 20 bp were designed and synthesized for SCAR-PCR. The result of the SCAR-PCR amplification showed that one of the special bands, the molecular size was 649 bp, did not exist actually. There was a piece of special band with the molecular size of 740 bp in the SCAR-PCR of Wancui, but not in that of Hayward. Therefore the special band with 740 bp had been proved to be true DNA fragment between Wancui and Hayward. RAPD marker of genetic variation in Wancui had been transformed to SCAR marker successfully, it could be used for cultivar rapid identification.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2007年第2期251-255,共5页
Journal of Anhui Agricultural University
基金
安徽省高校青年教师科研计划(2006jql121)
安徽农业大学校长基金项目共同资助
关键词
皖翠
芽变位点
SCAR标记
Actinidia deliciosa cv. Wancui
sport site
SCAR marker
