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ARDRA联合RAPD对不动杆菌基因型鉴定的研究 被引量：4

Combination of ARDRA and RAPD Genotyping Technique in Acinetobacter spp.Genomic Species Identification
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摘要 收集多重耐药的不动杆菌10株,以标准参照株作对照,采用扩增核糖体DNA限制性酶切(ARDRA)DNA指纹技术联合随机扩增多态性DNA(RAPD)技术对其基因亚型进行分析;以非加权组间平均法(UPG-MA)进行聚类分析。该法可以有效地鉴定不动杆菌基因亚型;并从10株不动杆菌中鉴定出1株琼氏不动杆菌及9株鲍曼不动杆菌。ARDRA联合RAPD基因指纹分型技术有良好的互补性,可准确鉴定不动杆菌基因型。 A total of 10 nonrepetitive multidrug-resistant Acinetobacter strains were collected. With the reference of A, calcoaceticus （ ATCC23055 ） , A. baumanrdi （ATCC19606） A. lwoffii （ ATCC17986 ） and A. junii （ NCTC5866 ） , DNA fingerprint techniques ARDRA （ Amplified ribosomal DNA restriction Analysis） and RAPD （ Random amplified polymorphism DNA） were carried out to identify the genomic species of Acinetobacter spp. The distances between them were calculated by unweighted pair group method with arithmetic （UPGMA） . Genotypes of Acinetobacter spp. can be effectively classified and an A. junii together with nine A. baumannii isolates was genomically identified. The combination of ARDRA and RAPD DNA-fingerprint technique showed high complementarity, it could be a useful tool in Acinetobarter genotnic species identification.
作者 张永 陈余清 唐英春 张扣兴
机构地区 蚌埠医学院附属医院 中山大学附属第三医院
出处 《微生物学通报》 CAS CSCD 北大核心 2007年第2期303-306,共4页 Microbiology China
基金 安徽省高校自然科学研究项目(No2006kj49B)
关键词 不动杆菌 DNA指纹分型 随机扩增多态性 聚类分析 Acinetobacter, DNA Fingerprinting, RAPD, Cluster analysis
分类号 R378 [医药卫生—病原生物学]
  • 相关文献

参考文献13

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二级参考文献16

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共引文献5

同被引文献47

  • 1张永,唐英春,张扣兴,陆坚,莫晓能,潘晓娴,席云.琼氏不动杆菌对碳青霉烯类抗生素耐药分子机制研究[J].中国抗感染化疗杂志,2005,5(2):77-82. 被引量：6
  • 2张文君,严小军,郑立,叶芳挺,徐继林.利用脂肪酸组成分析鉴定海洋细菌的初步研究[J].科技通报,2006,22(4):462-466. 被引量：4
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引证文献4

二级引证文献12

微生物学通报
2007年 第2期

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