摘要
目的研究绍兴市中心医院ICU住院患者临床分离的耐碳青霉烯类鲍氏不动杆菌的耐药性和基因分型。方法收集2016年绍兴市中心医院ICU住院患者临床分离的耐碳青霉烯类鲍氏不动杆菌60株。微量稀释法进行常规药敏试验。采用多重PCR技术检测菌株携带的耐药基因,PFGE分型技术对鲍氏不动杆菌进行基因同源性分析。结果抗菌药物敏感性试验显示所有菌株具有多药耐药性,对哌拉西林、哌拉西林/他唑巴坦、头孢他啶、头孢吡肟耐药率>90%,对阿米卡星的敏感率较高为62.4%,其次为头孢哌酮/舒巴坦和左氧氟沙星;PCR检出60株鲍氏不动杆菌均为OXA-23型和OXA-51型。PFGE分型均为同一型别。结论绍兴市中心医院ICU分离的耐碳青霉烯鲍氏不动杆菌的耐药基因为OXA-23型和OXA-51型,为同一来源,考虑为医院感染所致,因此应采取有效措施来控制该耐药株的传播。
OBJECTIVE To investigate the drug resistance and genotypes of carbapenem-resistant Acinetobacter baumannii strains isolated from the patients who were hospitalized in the ICUs of the Centre Hospital Shaoxing City Zhejiang Province.METHODS A total of 60 strains of carbapenem-resistant A.baumannii were isolated from the patients who were hospitalized in the ICUs of the Centre Hospital Shaoxing City Zhejiang Province,the routine drug susceptibility testing was performed by using microdilution method,the drug resistance genes that were carried by strains were detected by means of multiple PCR technique,and the homology of the genes was analyzed with the use of PFGE.RESULTS The result of the drug susceptibility testing indicated that all of the strains were multidrug-resistant,the drug resistance rates to piperacillin,piperacillin-tazobactam,ceftazidime and cefepime were more than 90%;the drug susceptibility rate to amikacin was the highest(62.4%),followed by cefoperazonesulbactam and levofloxacin.The PCR result showed that all of the 60 strains of A.baumannii were detected to carry with OXA-23 and OXA-51 genotypes;the PFGE result showed that all of the strains carry with the same genotype.CONCLUSION OXA-23 and OXA-51 genotypes are detected in the carbapenem-resistant A.baumannii strains isolated from the ICUs of the Centre Hospital Shaoxing City Zhejiang Province and are the homological genotype,which is considered to be induced by nosocomial infection,therefore,it is necessary to take effective measures to control the spread of the strains.
作者
陈海潮
赵锋
李三验
CHEN Hai-chao;ZHAO Feng;LI San-yan(The Centre Hospital Shaoxing City Zhejiang Province,Shaoxing,Zhejiang 312030,China)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2019年第1期6-9,共4页
Chinese Journal of Nosocomiology
基金
浙江省卫生厅基金资助项目(2014KYB205)
关键词
碳青霉烯酶
鲍氏不动杆菌
耐药分析
基因分型
Carbapenemase
Acinetobacter baumannii
Drug resistance analysis
Genotyping
