摘要
利用随机扩增多态性(RAPD-PGR)的方法对48株白念珠菌Candida albicans(Robin)Berkh进行了分析。由初步试验中,随机选用了18种引物,筛选出OPA-14引物,该引物扩增的带型清晰可辨,不同菌株之间扩增的带数6—12条不等,共有6条主带。扩增片段的长度粗略估计在300—2000bp左右。除个别菌株的带型相同以外,大多数菌株之间呈多态性分布,其带型的数目和扩增的片段存在差异。对简单的DNA制备方法,以及RAPD-PCR在临床应用和流行病学调查中的可行性进行了初步探讨。
Randomly amplified polymorphic DNA markers (RAPD-PCR) analysis-was performed in 46 clinic strains and 2 standard strains of Candida albicans. Only the profiles of OPA-14 derived from 18 random primers were very distict. It showed that the amplified numbers of band were from 6 to 12 among different strains. However, there were 6 identical fragments that can be observed in all the amplified profiles of strains, the length of fragments varied from 300 to 2 000bp. Most of strains showed a wide diversity in number and length of amplified fragments. In this paper, the feasibility of application of RAPD-PCR and isolation of fungal DNA by means of benzyl chloride in clinic and epidemiology were also discussed.
出处
《真菌学报》
CSCD
北大核心
1995年第2期123-129,共7页
基金
卫生部科学研究基金
关键词
白色念珠菌
分型
随机扩增多态性
病原真菌
Candida albicans, Classification, Randomly amplified polymorphic DNA markers
