摘要
目的菝葜属百合科植物,为一种常用中药,目前所用的菝葜提取物多为其40%的乙醇提取物,本实验室首创了菝葜的乙酸乙酯提取工艺并对其进行了抗癌活性研究,旨在探讨其抗肿瘤作用及机制。方法流式细胞仪分析菝葜乙酸乙酯提取物对肿瘤细胞细胞周期的影响;用荧光显微镜和琼脂糖凝胶电泳观察菝葜乙酸乙酯提取物诱导细胞凋亡的作用。结果菝葜乙酸乙酯提取物可有效抑制肿瘤细胞的增殖,并通过对肿瘤细胞直接杀伤和促进肿瘤细胞凋亡来发挥作用。结论菝葜乙酸乙酯提取物具有抗肿瘤作用,有进一步开发的价值。
Objective Chinese herb Smilax China L （SCL） is a liliaceous plant which is in common use. Now, the preparations of Smilax are usually extracted by 40% ethanol, we were invented a new means to extract the effective material in Smilax and made much research on it＇ s action of anti-cancer. This study was designed to investigate the anti-cancer activity of SCL extract by ethylacetate and the related mechanism . Methods The effect of SCL extract on tumor cells proliferation, cell cycle distribution and apoptosis inducing were determined by mouse model and cell molecular biological methods including MTT assay, agarose gel electrophoresis, flow cytometry and Hoechst 33342/PI fluorescent staining, Results SCL extract showed proliferation inhibition on several mouse tumor cells and HepG2 cells by kill tumor cells di- rectly and inducing apoptosis. Conclusions SCL showed proliferation inhibition on several tumor, and further studies on this effect will be proceeded.
出处
《肿瘤基础与临床》
2007年第2期129-131,共3页
journal of basic and clinical oncology
关键词
菝葜
抗肿瘤
细胞凋亡
Smilax China L
anti-tumor
apoptosis
