摘要
目的:研究奥曲肽(octreotide,OCT)对人胃癌细胞株MKN45生长的调控作用,并探讨其作用机制。 方法:采用MTT比色分析法测定OCT10^(-2),10^(-3),10^(-4),10^(-5),10^(-6)g·L^(-1)对MKN45细胞生长的调控作用;采用流式细胞术分析OCT10^(-3)g·L^(-1))对MKN45细胞的周期分布的影响。 结果;OCT 10^(-2),10^(-3),10^(-4)g·L^(-1)对MKN45细胞的生长均有抑制作用,以lO^(-3)g·L^(-1)浓度的抑制作用最显著,为10.4％;10^(-3)g·L^(-l)这一浓度可诱导MKN45出现G0/G1阻滞,于加入OCT后6h开始,为(64±4)％,24h最显著,达(75±3)％,36h已消失;与对照组(7±1)％相比,24h G2/M期细胞比例亦减少,为(2±2)％,但OCT未改变亚二倍体细胞的比例。 结论:OCT可抑制人胃癌细胞株MKN45的生长,其作用机制之一是诱导细胞出现GO/G1阻滞。
AIM: To study the effects and mechanisms that the somatostatin analog octreotide (OCT) exerted on the growth of human gastric cancer cell MKN45.METHODS: MTT colorimetric assay and flow cytometric analysis of DNA histograms were used to measure the proliferation and cell cycle distribution of MKN45 cells.RESULTS: OCT of 10-2, 10-3 and 10-4 g · L-1 exerted the inhibitory actions on MKN45 cells, and the maximum inhibitory rate was 10.4% by 10-3 g·L-1OCT. OCT of 10-3g· L-1 also induced MKN45 to G0/G1 arrest,which began at 6h (64±4)% after OCT was added, and peaked at 24h(75± 3)%,then disappeared at 36h. At 24h, the percentage of G2/M cells also decreased to 2 ± 2% as compared with the controls(7 ±1%). OCT did not change the percentage of hypodiploid cells.CONCLUSIONS: Inhibition of somatostatin analog octreotide on human gastric cancer cell MKN45 growth in vitro involves GO/Gl phase arrest.
出处
《世界华人消化杂志》
CAS
2002年第1期40-42,共3页
World Chinese Journal of Digestology
基金
江苏省科委(BJ98110)
江苏省卫生厅(H9808)资助课题
